| Project/Area Number |
26450298
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Fisheries Research and Education Agency |
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Principal Investigator |
Okuzawa Koichi 国立研究開発法人水産研究・教育機構, 増養殖研究所, センター長 (00211813)
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| Co-Investigator(Kenkyū-buntansha) |
宇治 督 国立研究開発法人水産研究・教育機構, 増養殖研究所, 主任研究員 (40372049)
風藤 行紀 国立研究開発法人水産研究・教育機構, 増養殖研究所, グループ長 (60399996)
藤原 篤志 国立研究開発法人水産研究・教育機構, 中央水産研究所, 主幹研究員 (30443352)
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| Research Collaborator |
SATOH Mana
NYUJI Mitsuo
YAMAGUCHI Toshiya
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 卵成熟 / 卵成熟能 / マダイ / トランスクリプトーム / 卵濾胞 / ステロイドホルモン / 遺伝子発現 / hCG / 卵成熟誘起ステロイド / ゲノム / 次世代シークエンサー / 半数体 |
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| Outline of Final Research Achievements |
Transcriptomes were compared between maturationally competent and incompetent ovarian follicles of red seabream, Pagrus major. Gene expressions of luteinizing hormone receptor and progesterone receptors of competent follicles were 8 and 6 times higher than incompetent ones, respectively. An artificial induction of the competency by human chorionic gonadotropin (hCG) resulted in up-regulations of the genes encoding enzymes catalyzing sex steroid hormone syntheses, tight junction protein, etc. Among them, a putative gene with enhanced gene expression of around 900-fold by hCG is estimated as the key enzyme catalyzing production of maturation-inducing steroid.
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