| Project/Area Number |
26450412
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丸山 総一 日本大学, 生物資源科学部, 教授 (30181829)
横山 栄二 千葉県衛生研究所, 細菌研究室, 室長 (40370895)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 志賀毒素産生大腸菌 / 鹿 / ジビエ / 食中毒 / 猪 / O157 / ゲノム解析 |
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| Outline of Final Research Achievements |
Shiga toxin producing Escherichia coli (STEC) and STEC O157 were isolated from 38 (11.8%) (49 strains) and 9 (2.8%)(9 strains) out of 323 deer in Japan. Some strains contained several pathogenicity-related genes, such as stx, eaeA and hlyA. Phylogenetic analysis of the STEC O157 strains demonstrated that one and eight strains were grouped in Clades 7 and 12, respectively. The clades 7 and 12 included some strains derived from human patients with hemorrhagic- and watery- diarrhea, respectively.
Thirty-one STEC strains derived from deer were used for the phylogenetic analysis based on the patterns of the presences of 24 pathogenicity-related genes and it was revealed that 2 strains of O5 STEC were significantly related to the strains derived from human patients, suggesting the possibility that the deer strains could show pathogenicity to humans.
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