| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
It was revealed that canine corneal epithelial cells can maintain their undifferentiated state and proliferation ability even when cultured without any feeder cells. Thus, feeder cells can be eliminated for fabrication of canine corneal epithelial cell sheets. However, canine corneal epithelial cells showed apparent morphological change when cultured using autologous serum. Therefore, the use of fetal bovine serum could not be eliminated to fabricate canine corneal epithelial cell sheets. In the process to explore the possible growth factors to fabricate canine corneal epithelial cells, we found out that hepatocyte growth factor (HGF) promotes the cell invasion ability of canine corneal epithelial cells and HGF can be a promising drug to treat corneal injury. We could also developed transplantation of canine corneal epithelial cell sheets without sutures.
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