Myeloperoxidase negatively regulates the expression of proinflammatory cytokines and chemokines by mouse neutrophils
Project/Area Number |
26450446
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Integrative animal science
|
Research Institution | Yokohama City University |
Principal Investigator |
Aratani Yasuaki 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (30192470)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 免疫 / 好中球 / ミエロペルオキシダーゼ / 活性酸素 / 炎症 / 食細胞NADPHオキシダーゼ / 自然免疫 |
Outline of Final Research Achievements |
Myeloperoxidase (MPO) and NOX2, major constituents of neutrophils that generate reactive oxygen species (ROS), contribute to microbial killing. This study aimed to evaluate the effect of MPO deficiency and NOX2 deficiency on lung inflammation induced by zymosan and nonviable Candida. Mice deficient in these enzymes showed more severe neutrophilic pneumonia than wild-type mice, which exhibited higher lung concentrations of proinflammatory cytokines chemokines. In vitro, production of these inflammatory mediators from neutrophils was enhanced in the mutant neutrophils, concomitant with upregulation of ERK/NF-κB pathway. We also found that upregulation of the CD11b/FAK/ERK pathway due to absence of MPO enhances the phagocytic activity of neutrophils. These results suggest that lack of ROS production results in the production of higher levels of proinflammatory mediators. Thus, loss of ROS production by neutrophils causes significant abnormalities in both host defense and inflammation.
|
Report
(4 results)
Research Products
(27 results)
-
[Journal Article] Genetic phagocyte NADPH-oxidase deficiency enhances nonviable Candida albicans-induced inflammation in mouse lungs.2017
Author(s)
Endo, D., Fujimoto, K., Hirose, R., Yamanaka, H., Homme, M., Ishibashi, K., Miura, N., Ohno, N., and Aratani, Y
-
Journal Title
Inflammation
Volume: 40
Issue: 1
Pages: 123-135
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
[Journal Article] The potential for repositioning antithyroid agents as antiasthma drugs.2016
Author(s)
Suzuki S, Ogawa M, Ohta S, Arima K, Nunomura S, Nanri Y, Mitamura Y, Yoshihara T, Nakamura Y, Yamauchi K, Chibana K, Ishii Y, Lee JJ, Aratani Y, Kakuta S, Kubo S, Iwakura Y, Yoshida H, Izuhara K.
-
Journal Title
J Allergy Clin Immunol.
Volume: 138
Issue: 5
Pages: 1458-1461
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
[Presentation] Voltage-gated proton channel Hv1/VSOP inhibits granule exocytosis in neutrophils and inflammation.2015
Author(s)
Okochi, Y., Aratani, Y., Adissu, H., Miyawaki, N., Sasaki, M., Suzuki, K., and Okamura, Y
Organizer
8th FAOPS Congress
Place of Presentation
Bangkok(Thailand)
Year and Date
2015-11-22
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-