| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Rab proteins are small monomeric GTPases/GTP- binding proteins and form the largest branch of Ras super-family. RabX4 and RabX6 are an insect-specific Rab proteins that have no close homolog in vertebrates. RabX4-like immunohistochemical reactivity (RabX4-ir) was restricted to neurons of the pars intercerebralis and dorsolateral protocerebrum in the brain. Corpus allatum is a neuronal organ to secrete neuropeptides synthesized in the brain into the hemolymph. In corpus allatum, RabX4-ir was co-localized with the Rab6-ir. RabX6-like immunohistochemical reactivity (RabX6-ir) was identified at the face of the testis, not in the spermatogonia, and was specifically detected at a pair of tritocerebral cells of the male brain. Furthermore, RNA interference of RabX6 was shown to decrease testicular growth. Double staining analysis of Rabs and insulin-like peptide, bombyxin indicate that 4 Rabs-ir are overlapped with bombyxin-ir in the brain and corpus allatumn of Bombyx mori.
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