| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this study, we created Nestin-cre Ubiad1-CKO mice which were Ubiad1 deficient from the time of neural stem cell. Nestin-cre Ubiad1-CKO was born normally, but these mice were slightly smaller than that of the wild type mice. We measured the Ubiad1 expression and MK-4 concentration in brain tissue at 10 weeks of age. Ubiad1 expression was remarkably low or below the detection limit in any brain tissues. MK-4 concentration was also remarkably low or below the detection limit. We evaluated the brain functions by behavior analysis for 9 to 10 week old Nestin-cre Ubiad1-CKO mice. As a result, short-term memory, anxiety and motor coordination was decreased in Nestin-cre Ubiad1-CKO mice. Moreover, the brain tissue of Nestin-cre Ubiad1-CKO mouse revealed cerebellar atrophy and disappearance of cerebellar Purkinje cells. Therefore, it was suggested that UBIAD1 and MK-4 play an essential role in maintaining structural and function in the cerebellar.
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