Determination of conjugated steroids in dried urine spots by means of liquid chromatography-mass spectrometry

• Project/Area Number: 26460211
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kindai University
• Principal Investigator: MITAMURA Kuniko 近畿大学, 薬学部, 准教授 (70242526)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 乾燥ろ紙尿 / LC/MS / テトラヒドロコルチコステロイド / オキシステロール / グルクロン酸抱合体 / 硫酸抱合体 / 乾燥尿ろ紙 / 抱合体 / コレステロール / 尿中代謝物

Outline of Final Research Achievements

Profile determination methods for oxysterols sulfate and tetrahydrocorticosteroids glucuronides (THC-G) and/or these sulfates in urine samples by means of LC/MS/MS were developed. The method was applicable to quantitative analysis of THC-G in dried urine spots (DUS) sample. The method was well validated, and the concentration of THC-G in DUS were well correlated with those in liquid urine. DUS samples provides convenient and safe transportation, storage and handling of sample. DUS samples would be a useful alternative for clinical screening tests of endocrine disorders, particularly, when the sample needs to be transported over long distances.

Research Products

• [Journal Article] Two Major Bile Acids in the Hornbills, (24R,25S)-3α,7α,24-Trihydroxy-5β-cholestan-27-oyl Taurine and Its 12α-Hydroxy Derivative (2016)
  • Author(s): R.Satoh, H.Ogata, T.Saito, B.Zhou, K.Omura, S.Kurabuchi, K.Mitamura, S.Ikegawa, L.R.Hagey, A.F.Hofmann, T.Iida.
  • Journal Title: Lipids Volume: 51 Issue: 6 Pages: 757-768
  • DOI: 10.1007/s11745-016-4150-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue. (2016)
  • Author(s): Yamamoto T, Kudo M, Peng WX, Takata H, Takakura H, Teduka K, Fujii T, Mitamura K, Taga A, Uchida E, Naito Z
  • Journal Title: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine Volume: 37 Issue: 10 Pages: 13595-13606
  • DOI: 10.1007/s13277-016-5275-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 2.Novel, major 2α- and 2β-hydroxy bile alcohols and bile acids in the bile of Arapaima gigas, a large South American river fish. (2016)
  • Author(s): Sato Née Okihara R, Saito T, Ogata H, Nakane N, Namegawa K, Sekiguchi S, Omura K, Kurabuchi S, Mitamura K, Ikegawa S, Raines J, Hagey LR, Hofmann AF, Iida T.
  • Journal Title: Steroids Volume: 107 Pages: 112-120
  • DOI: 10.1016/j.steroids.2016.01.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 3.Improvement of solid material for affinity resins by application of long PEG spacers to capture the whole target complex of FK506 (2015)
  • Author(s): Mabuchi M, Shimizu T, Ueda M, Mitamura K, Ikegawa S, Tanaka A.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 25 Issue: 14 Pages: 2788-2792
  • DOI: 10.1016/j.bmcl.2015.05.014
  • Peer Reviewed
• [Journal Article] 3.Development and validation of a method for determination of plasma 25-hydroxyvitamin D3 3-sulfate using liquid chromatography/tandem mass spectrometry. (2014)
  • Author(s): Higashi, T, Goto, A, Morohashi, M, Ogawa, S, Komatsu, K, Sugiura, T, Fukuoka, T, Mitamura, K
  • Journal Title: J. Chromatogr. B Volume: 969 Pages: 230-234
  • DOI: 10.1016/j.jchromb.2014.08.027
  • Peer Reviewed
• [Presentation] ICD-LC/ESI-MS/MSによる尿中テトラヒドロコルチコステロイドグルクロニド定量法の開発 (2017)
  • Author(s): 松本孝彬、山崎航、小川祥二郎、三田村邦子、池川繁男、東達也
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台国際センター（仙台市）
  • Year and Date: 2017-03-25
• [Presentation] 細胞外基質 Lumican の発現抑制による新規膵臓癌細 胞増殖抑制法の開発 (2017)
  • Author(s): 山本 哲志, 谷田 和香奈, 橋本 知樹, 三田村 邦子, 多賀 淳
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台国際センター（仙台市）
  • Year and Date: 2017-03-25
• [Presentation] Identificaiton of antitumor component in maple syrup to develop novel anti-cancer drugs for colorectal cancer. (2016)
  • Author(s): Yamamoto T, Shiburo R, Mitamura K, Taga A.
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] The role of maple syrup on cell proliferation of colorectal cancer cells. (2016)
  • Author(s): Kubota C, Yamamoto T, Mitamura K, Taga A.
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] Protein component in maple syrup has a potential to develop novel anti-cancer drugs for colorectal cancer. (2016)
  • Author(s): Yamamoto T, Shiburo R, Mitamura K, Taga A.
  • Organizer: 米国消化器病週間（DDW2016)
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-05-21
  • Int'l Joint Research
• [Presentation] The role of cyclophilin A as a novel therapeutic target of colorectal cancer based on proteome analysis using formalin-fixed and paraffin embedded colorectal cancer tissue. (2016)
  • Author(s): Takakura H, Yamamoto T, Mitamura K, Kudo M, Naito Z, Taga A.
  • Organizer: 米国消化器病週間（DDW2016)
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-05-21
  • Int'l Joint Research
• [Presentation] LC/ESI-MSによる乾燥ろ紙尿中糖質コルチコイド代謝物のスクリーニング法の開発 (2016)
  • Author(s): 三田村 邦子、森 莉子、上田麻美子、亀井美希、山本哲志、多賀 淳
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜（パシフィコ横浜）
  • Year and Date: 2016-03-27
• [Presentation] 薬学による臨床化学へのアプローチ (2015)
  • Author(s): 三田村邦子
  • Organizer: 第55回日本臨床化学会年次学術集会
  • Place of Presentation: 大阪（大阪大学コンベンションセンター）
  • Year and Date: 2015-10-30
• [Presentation] Comparison of the sensitivity and selectivity for the CID and PQD mode in the analysis of conjugated steroids (2015)
  • Author(s): Kuniko Mitamura, Satoshi Kurabuchi, Mamiko Ueda, Tetsushi Yamamoto, Atsushi Taga, Shigeo Ikegawa
  • Organizer: 63th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: St. Louis, Missouri (America's Center)
  • Year and Date: 2015-05-31
  • Int'l Joint Research
• [Presentation] LC/MSによる抱合型ステロイド代謝物測定～臨床への応用を目指して～ (2015)
  • Author(s): 三田村邦子
  • Organizer: 日本分析化学会近畿支部2015年度第1回講演会
  • Place of Presentation: 大阪（大阪科学技術センター）
  • Year and Date: 2015-04-10
  • Invited
• [Presentation] 2.LC/ESI-MS/MSによる尿中抱合型テトラヒドロコルチコステロイドのプロファイル分析 (2015)
  • Author(s): 三田村邦子、上田麻美子、山本哲志、多賀淳、池川繁男
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸サンボーホール（兵庫県神戸市）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 5.LC/ESI-LIT-MS/MSにおけるPQD及びCID測定による尿中硫酸抱合型ステロイドの分析 (2014)
  • Author(s): 三田村邦子、蔵渕 慧、上田麻美子、池川繁男、山本哲志、多賀 淳
  • Organizer: 第39回日本医用マススペクトル学会年会
  • Place of Presentation: 三井ガーデンホテル千葉（千葉県千葉市）
  • Year and Date: 2014-10-16 – 2014-10-17
• [Presentation] 8.同位体希釈LC/ESI-MS/MSによるヒト尿中硫酸抱合型オキシステロールの高感度直接一斉定量法の開発 (2014)
  • Author(s): 蔵渕 慧、三田村邦子、池川繁男、山本哲志、多賀淳
  • Organizer: 第64回日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都薬科大学（京都府京都市）
  • Year and Date: 2014-10-11
• [Presentation] 6.LC/ESI-MS/MSによる尿中抱合型テトラヒドロコルチコステロイドのプロファイル分析－硫酸抱合体とグルクロン酸抱合体の分別定量の試み－ (2014)
  • Author(s): 上田麻美子、三田村邦子、山本哲志、多賀淳
  • Organizer: 第64回日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都薬科大学（京都府京都市）
  • Year and Date: 2014-10-11