| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
This study was aimed at elucidating the role of dendritic cells (DCs) on HIV-1 transmission in vivo using humanized mice. Because the development of myeloid-lineage immune cells including DCs is poor in conventional humanized mice, introduction of human GM-CSF and Flt3-L into humanized mice was tested to induce DCs. As a result, two myeloid DC subsets, BDCA-1+ MDC1 and BDCA-3+ MDC2, were significantly induced by treatment with these cytokines. When these DC-induced humanized mice and control humanized mice were infected with HIV-1, a significant correlation between the absolute cell number of blood monocytes pre-infection and the plasma viral load at 1 week post-infection was observed. This result suggests that DCs are involved in the efficacy of HIV-1 transmission in vivo at the early phase of infection.
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