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Characterization of the gut microbiota of Kawasaki disease patients by metagenomic analysis

Research Project

Project/Area Number 26460542
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Kuroda Makoto  国立感染症研究所, 病原体ゲノム解析研究センター, センター長 (80317411)

Research Collaborator OBA Kunihiro  公立昭和病院, 小児科, 医長
HAMADA Hiromichi  東京女子医大八千代医療センター, 小児科
SEKIZUKA Tsuyoshi  国立感染症研究所, 病原体ゲノム解析研究センター, 室長
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords川崎病 / レンサ球菌 / メタゲノム解析 / 腸内フローラ / メタゲノム / 感染症
Outline of Final Research Achievements

Kawasaki disease (KD) is an acute febrile illness of early childhood. Additionally, previous culture-based studies have suggested a possible role of intestinal microbiota in KD pathogenesis. In this study, we performed metagenomic analysis to comprehensively assess the longitudinal variation in the intestinal microbiota of 28KD patients. Several notable bacterial genera were commonly extracted during the acute phase, whereas a relative increase in the number of Ruminococcus bacteria was observed during the non-acute phase of KD. The metagenomic analysis results based on bacterial species classification suggested that the number of sequencing reads with similarity to five Streptococcus spp. (S. pneumonia, pseudopneumoniae, oralis, gordonii, and sanguinis), in addition to patient-derived Streptococcus isolates, markedly increased during the acute phase in most patients. The findings of this study suggest that KD-related Streptococci might be involved in the pathogenesis of this disease.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Comprehensive pathogen detection associated with four recurrent episodes of Kawasaki disease in a patient during a single year using next-generation sequencing.2016

    • Author(s)
      Hamada H, Sekizuka T, Oba K, Katano H, Kinumaki A, Terai M, Mizutani T, Kuroda M.
    • Journal Title

      JMM Case Rep.

      Volume: 1;3(1) Issue: 1

    • DOI

      10.1099/jmmcr.0.005019

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of the gut microbiota of Kawasaki disease patients by metagenomic analysis2015

    • Author(s)
      Akiko Kinumaki, Tsuyoshi Sekizuka, Hiromichi Hamada, Kengo Kato, Akifumi Yamashita, Makoto Kuroda
    • Journal Title

      Frontiers in Microbiology

      Volume: 6 Pages: 824-824

    • DOI

      10.3389/fmicb.2015.00824

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 次世代シークエンサーによって Streptococcus gallolyticus subsp. pasteurianus を検出した細菌性髄膜炎の5歳児の一例2015

    • Author(s)
      田村恵美、 名井栄実菜、 秋山聡香、 小花奈都子、 川口隆弘、 林健太、 野田雅裕、 吉田知広、大場邦弘、 小鍛治雅之、小田智三、 黒田誠
    • Organizer
      第47回日本小児感染症学会総会
    • Place of Presentation
      福島市
    • Year and Date
      2015-10-31
    • Related Report
      2015 Research-status Report
  • [Presentation] 川崎病患児の腸内細菌フローラ解析2015

    • Author(s)
      黒田誠, 関塚剛史
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜市長良福光2695-2 長良川国際会議場
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Human oral, gut, and blood microbiota in patients with Kawasaki disease2015

    • Author(s)
      Jun Abe, Rhota Ebata, Naoki Saito, Kentaro Okunushi, Kazuhiko Nakabayashi, Makoto Kuroda
    • Organizer
      IKDS 2015, 11th International Kawasaki Disease Symposium
    • Place of Presentation
      Hilton Hawaiian Village Honolulu, Hawaii
    • Year and Date
      2015-02-03 – 2015-02-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 川崎病患者の末梢血、咽頭・直腸スワブのメタゲノム解析2014

    • Author(s)
      阿部 淳、中林 一彦、江畑 亮太、黒田 誠
    • Organizer
      第34 回日本川崎病学会・学術集会
    • Place of Presentation
      学術総合センター2F 一橋講堂
    • Year and Date
      2014-10-31 – 2014-11-01
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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