Mechanism of production, secretion and hypertension in the endogenous digitalis like factors using mass spectrometry immunoassay
| Project/Area Number |
26460662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小宮山 豊 関西医科大学, 医学部, 講師 (40140264)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 内因性ジギタリス / 質量分析イムノアッセイ / 高血圧 / 生理活性物質 / 質量分析 / マリノブファギン / マリノブフォトキシン / テロシノブフォトキシン / ELISA / レニン / アルドステロン |
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| Outline of Final Research Achievements |
This research is a part of patho-physiological study of endogenous digitalis-like factors (EDLFs). We have marinobufagin, telocinobufagin, and their related compounds, maronobufotoxin and telicinobufotoxin. Moreover, we reported they are existed in human plasma. EDLFs have low monocular compounds with steroid ring. Therefore their analytic methods have only some techniques. Recently MISA, which has been interested by same field’s researchers, can be a technique for high sensitive qualitative as well as quantitative analysis in lesser sample volunme. We tried MISA was used more easily and high specific pretreatment of our ELISA instead of C18 seppak columns. Our each antibodies was absorbed in MISA tubes, and then samples was added in MISA tube. In this tube, we tried to extract only target substances.
Unfortunately, the adequate antigen‐antibody reactionin was not achieved in this study.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] ラテックス凝集法とEIA法で血小板第4因子-ヘパリン複合体抗体の測定結果に乖離を生じたヘパリン起因性血小板減少症の1例2014
Author(s)
吉賀正亨、小宮山豊, 松本宙士, 平井雅美, 寺内理恵, 野村明代, 前羽宏史, 今田崇裕, 大倉ひろ枝, 角坂芳彦, 塩島一朗, 高橋伯夫
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Journal Title
臨床病理
Volume: 62
Pages: 1047-1051
Related Report
Peer Reviewed
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