• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the mechanism responsible for the onset of ulcerative colitis using S100 transgenic rats

Research Project

Project/Area Number 26460663
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionTenri Health Care University

Principal Investigator

Ikemoto Masaki  天理医療大学, 医療学部, 教授 (80144385)

Co-Investigator(Kenkyū-buntansha) 戸田 好信  天理医療大学, 医療学部, 教授 (10444465)
竹田 真由  天理医療大学, 医療学部, 助教 (00423054)
仲瀬 裕志  京都大学, 医学研究科, 講師 (60362498)
岡田 光貴  天理医療大学, 医療学部, 助手 (80747569)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsマクロファージ / トランスジェニックラット / 急性炎症 / LPS / S100タンパク質 / 潰瘍性大腸炎 / 炎症性腸疾患 / Macrophage / Endocytosis / S100 proteins / IBD / neutrophil / nuclear / cytokines / inhibition / 免疫細胞 / オートクライン / サイトカイン
Outline of Final Research Achievements

We artificially inserted S100A8 cDNA to the pCXGFP vector and then injected the resultant plasmid to embryonic stem (ES) cells of rats, and conclusively succeeded in having a S100A8 transgenic rat (Tg-S100A8). Lipopolysaccharide (LPS) was intraperitoneally administrated in wild-type rats and Tg-S100A8, so that acute liver damage has been induced in the liver tissue of WT-rats, where many macrophages have been also accumulated. While, acute liver damage in Tg-S100A8 was not almost observed, and immune cells were scattered in the liver tissue. These facts suggest that S100A8 could serve as an anti-inflammatory protein in Tg-S100A8. We are attempting to elucidate the mechanisms responsible for negatively regulating acute liver damage by LPS in Tg-S100A8.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (17 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Autocrine pathways involving S100A8 and/or S100A9 that are postulated to to regulate the immunological functions of macrophages in rats2016

    • Author(s)
      Kohki Okada, Satoshi Arai, Hiroshi Nakase, Hisashi Kohno, Fumihiko Nakamura, Mayu Takeda, Yoshinobu Toda, Hiroshi Itoh, Souichi Adachi, Masaki Ikemoto
    • Journal Title

      BBRC

      Volume: 456 Pages: 415-420

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] CD68 on rat macrophages binds tightly to S100A8 and S100A9 and helps to regulate the cells’ immune functions2016

    • Author(s)
      Kohki Okada, Satoshi Arai, Hiroshi Itoh, Souichi Adachi, Hiroshi Nakase, Masaki Ikemoto
    • Journal Title

      J Leukoc Biol

      Volume: 100 Pages: 1093-1104

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Enzyme-linked immunosorbent assay for S100A9 in the stool of rats with dextran sulfate sodium-induced colitis.2016

    • Author(s)
      Shunsuke Sekiya, Makoto Murata, Satoshi Arai, Hiroshi Murayama, Atushi Kawasaki, Noriyuki Ashida, Kohki Okada, Masaki Ikemoto.
    • Journal Title

      J Immunol Methods

      Volume: 439 Pages: 44-49

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Autocrine pathways involving S100A8 and/or S100A9 that are postulated to to regulate the immunological functions of macrophages in rats2015

    • Author(s)
      Okada K, Arai S, Nakase H, Kohno H, Nakamura F, Takeda M, Toda Y, Itoh H, Adachi S, Ikemoto M
    • Journal Title

      Biochem Biophys Res Commun (BBRC)

      Volume: 456 Pages: 415-420

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Autocrine pathways involving S100A8 and/or S100A9 that are postulated to regulate the immunological functions of macrophages in rats2015

    • Author(s)
      Kohki Okada, Satoshi Arai, Hiroshi Nakase, Hisashi Kohno, Fumihiko Nakamura, Mayu Takeda, Yoshinobu Toda, Hiroshi Itoh, Souichi Adachi, Masaki Ikemoto1
    • Journal Title

      Biochemical and Biophysical Research Communication

      Volume: 456 Pages: 415-420

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 炎症性腸疾患における血清S100A8/A9のバイオマーカーとしての意義2016

    • Author(s)
      岡田光貴,荒井 智,関屋俊介,池本正生
    • Organizer
      第56回日本臨床化学会年次学術集会
    • Place of Presentation
      くまもと県民交流館パレア
    • Year and Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] S100タンパク質によるCD68を介したマクロファージ活性化機構に関する研究2016

    • Author(s)
      岡田光貴,関屋俊介,池本正生
    • Organizer
      第38回日本分子生物学会年会,第88回日本生化学会大会の合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2016-12-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] CD68を介したS100A8及びS100A9によるマクロファージ活性化機構に関する研究2016

    • Author(s)
      岡田光貴,荒井智,戸田好信, 竹田真由,仲瀬裕志,池本正生
    • Organizer
      第61回日本臨床検査医学会学術集会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-11-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] LPS誘導急性肝障害ラットにおけるS100タンパク質の免疫機能に関する研究2016

    • Author(s)
      岡田光貴,関屋俊介,戸田好信, 池本正生
    • Organizer
      第62回日本臨床検査医学会学術集会
    • Place of Presentation
      長良川国際会議場・岐阜都ホテル
    • Year and Date
      2016-11-19
    • Related Report
      2016 Annual Research Report 2015 Research-status Report
  • [Presentation] DSS誘導潰瘍性大腸炎ラットの血清中において変動するタンパク質の同定及びその臨床的意義について2016

    • Author(s)
      岡田光貴,荒井 智,関屋俊介,池本正生
    • Organizer
      第55回日本臨床化学会年次学術集会
    • Place of Presentation
      大阪大学コンベンションセンター
    • Year and Date
      2016-10-30
    • Related Report
      2016 Annual Research Report 2015 Research-status Report
  • [Presentation] ラットマクロファージ免疫機構におけるS100A8及びS100A9のオートクライン的制御経路の可能性に関する研究2016

    • Author(s)
      岡田光貴,荒井智,戸田好信, 竹田真由,仲瀬裕志,池本正生
    • Organizer
      第54回日本臨床化学会年次学術集会
    • Place of Presentation
      東京大学本郷キャンパス
    • Year and Date
      2016-09-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] S100A8及びS100A9を指標とした潰瘍性大腸炎発症メカニズムの解析に関する研究2016

    • Author(s)
      岡田光貴,荒井智,戸田好信, 竹田真由,仲瀬裕志,池本正生
    • Organizer
      第53回日本臨床化学会年次学術集会
    • Place of Presentation
      あわぎんホール
    • Year and Date
      2016-08-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] CD68 on rat macrophages binds tightly to S100A8 and S100A9, and subsequently helps to regulate the cell’s immune functions2016

    • Author(s)
      Okada K, Arai S, Itoh H, Adachi S, Nakase H, and Ikemoto M
    • Organizer
      21st IFCC-EFLM European Concress of Clinical Chemistry and Laboratory Medicine
    • Place of Presentation
      Paris Maillot, France
    • Year and Date
      2016-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] S100タンパク質によるCD68を介したマクロファージ活性化機構に関する研究2015

    • Author(s)
      岡田光貴,関屋俊介,池本正生
    • Organizer
      第38回日本分子生物学会年会と第88回日本生化学会大会の合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸国際会議場)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] CD68 on rat macrophages binds tightly to S100A8 and S100A9, and subsequently helps to regulate the cell’s immune functions2015

    • Author(s)
      Okada K, Arai S, Itoh H, Adachi S, Nakase H, and Ikemoto M
    • Organizer
      21st IFCC-EFLM European Concress of Clinical Chemistry and Laboratory Medicine
    • Place of Presentation
      France, Paris (Palais des Congres)
    • Year and Date
      2015-06-21
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] CD68を介したS100A8及びS100A9によるマクロファージ活性化機構に関する研究2014

    • Author(s)
      岡田光貴, 荒井智, 戸田好信, 竹田真由, 仲瀬裕志, 池本正生
    • Organizer
      第61回日本臨床検査医学会学術集会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2014-11-22 – 2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] ラットマクロファージ免疫機能におけるS100A8及びS100A9のオートクライン的制御経路の可能性に関する研究2014

    • Author(s)
      岡田光貴, 荒井智, 竹田真由, 戸田好信, 仲瀬裕志, 池本正生
    • Organizer
      第54回日本臨床化学会年会
    • Place of Presentation
      東京大学本郷キャンパス
    • Year and Date
      2014-09-05 – 2014-09-07
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi