| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
TRPV1 is activated by capsaicin. We applied 10 mM capsaicin-immersed cotton to the whisker pad for up to 6 days. Electron microscopic analysis revealed that there was extensive destruction of mitochondrial internal structure in small-sized trigeminal ganglion (TG) neurons at Day 4. Nevertheless, no discernible structural alterations were not observed in the small-sized TG neurons at Day 6, implying that there was a restorative process that normalized mitochondrial status in TG neurons exposed to repetitive TRPV1 stimulation. We established a PC12 clone stably expressing TRPV1. When exposed to capsaicin, these cells exhibited mitochondrial structural derangements in a dose-dependent manner. There were significantly increased mitophagy-affected mitochondria in capsaicin-treated cells. Our-cell-based assays imply that mitophagy plays an important role in the reparative process of mitochondrial health in capsaicin-treated cells.
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