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Estimates of elapsed time and personal identification from human blood sucked by mosquitoes

Research Project

Project/Area Number 26460885
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Legal medicine
Research InstitutionSaitama Medical University

Principal Investigator

HARA Masaaki  埼玉医科大学, 医学部, 准教授 (50129160)

Co-Investigator(Kenkyū-buntansha) 永井 淳  岐阜大学, 大学院医学系研究科, 准教授 (00207961)
田村 明敬  大阪医科大学, 医学部, 助教 (50207239)
山本 敏充  名古屋大学, 医学系研究科, 准教授 (50260592)
Research Collaborator HIROSHIGE Yuuji  
OGAWA Hisae  
HIKITSUCHI Tomoyuki  
UMEDA Mitsuo  
KAWAJIRI Yumi  
NAKAYAMA Koji  
SUZUKI Koichi  
TAKADA Aya  
ISHII Akira  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords蚊 / STR / Y-STR / 個人識別 / 吸血経過時間推定 / DNA定量 / DNA定量 / 吸血後経過時間推定
Outline of Final Research Achievements

In warm and hot seasons, fed mosquitoes are often observed at crime scenes. This study attempted to estimate the time that elapsed since feeding from the degree of human DNA digestion in mosquito blood meals and also to identify the individual human sources of the DNA using genotyping in two species of mosquito: Culex pipiens pallens and Aedes albopictus.
The extracted DNA were quantified using a human DNA quantification and QC kit and were genotyped for 15 STRs and 17 Y-STRs using a commercial multiplexing kits. It took about 3 days for the complete digestion of a blood meal, and genotyping was possible until 2 days post-feeding. The relative peak heights of the 15 STRs and DNA concentrations were useful for estimating the post-feeding time to approximately half a day between 0 and 2 days.
We suggest that this study may be very useful for estimating the time since a mosquito fed from blood meal DNA, although further refinements are necessary to estimate the times more accurately.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Acknowledgement Compliant: 1 results,  Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] 吸血した蚊からのY-STR型分析による個人識別2017

    • Author(s)
      永井 淳,原 正昭,廣重優二,小川久恵,引土知幸,梅田光夫,川尻由美,中山幸治,武内康雄,鈴木廣一,髙田 綾,石井 晃,山本敏充
    • Journal Title

      DNA多型

      Volume: 25

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Effects of storage conditions on forensic examinations of blood samples and bloodstains stored for 20 years.2016

    • Author(s)
      M.Hara, H.Nakanishi, K.Yoneyama, K.Saito, A.Takada
    • Journal Title

      Legal Medicine

      Volume: 18 Pages: 81-84

    • DOI

      10.1016/j.legalmed.2016.01.003

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationship between DNA degradation ratios and the number of loci detectable by STR kits in extremely old seminal stain samples.2015

    • Author(s)
      Masaaki Hara, Hiroaki Nakanishi, Shirushi Takahashi, Atsushi Nagai, Toshimichi Yamamoto, Katsumi Yoneyama, Kazuyuki Saito, Aya Takada
    • Journal Title

      Legal Medicine

      Volume: 17 Issue: 5 Pages: 391-393

    • DOI

      10.1016/j.legalmed.2015.05.008

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 吸血した蚊からのY-STR型分析による個人識別2016

    • Author(s)
      永井 淳,原 正昭,廣重優二,小川久恵,引土知幸,梅田光夫,川尻由美,中山幸治,武内康雄,鈴木廣一,髙田 綾,石井 晃,山本敏充
    • Organizer
      日本DNA多型学会第25回学術集会
    • Place of Presentation
      千葉 柏市 東京大学大気海洋研究所
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 吸血した蚊からの時間経過推定及びSTR型分析による個人識別(第1報)2016

    • Author(s)
      廣重優二,山本敏充,原 正昭,永井 淳,小川久恵,吉本高士,引土知幸,鈴木廣一,髙田 綾,石井 晃
    • Organizer
      第100次日本法医学会学術全国集会
    • Place of Presentation
      東京 品川区立総合区民会館
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effects of storage method on DNA degradation in old bloodstain samples2015

    • Author(s)
      M.Hara, H.Nakanishi, S.Takahashi, A.Tamura, K.Yoneyama, K.Saito, A.Takada
    • Organizer
      26rd Congress of the International Society for Forensic Genetics
    • Place of Presentation
      ポーランド,クラフク
    • Year and Date
      2015-09-03
    • Related Report
      2015 Research-status Report
  • [Presentation] Genetic polymorphisms at 17 Y-STR loci in an EGYPTIAN population2015

    • Author(s)
      A.Nagai, M.A. Elmadawy, T.Ishihara, T.Yamamoto, M.Hara, T. A. Yosef, G.M.Gomaa, H.M.R.Hegazy, Y.Bunai
    • Organizer
      26rd Congress of the International Society for Forensic Genetics
    • Place of Presentation
      ポーランド,クラフク
    • Year and Date
      2015-09-03
    • Related Report
      2015 Research-status Report
  • [Presentation] 陳旧精液斑からの個人識別に関する検討(第3報)-分解DNAサンプルにおけるSTR型判定の一考案-2015

    • Author(s)
      原正昭,中西宏明,木戸啓,高橋識志,齋藤一之,髙田綾,永井淳,米山克美,新井正明,冨山紀子
    • Organizer
      第99次日本法医学会学術全国集会
    • Place of Presentation
      高知市文化プラザかるぽーと(高知県高知市)
    • Year and Date
      2015-06-11
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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