| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Over the last decade, increasing evidence has suggested that the adult mammalian heart exhibits regenerative capacity due to proliferation of existing cardiomyocytes. It is unclear, however, if external stress may reactivate or upregulate the endogenous cardiac regenerative capacity. Acute left ventricle pressure overload was produced by constriction of ascending aorta (AAC) in adult mouse hearts. One week following AAC, ventricular afterload was normalized in a subset of animal through removal of the aortic constriction (de-AAC). Interestingly, acute decrease in ventricular overload with de-AAC was marked by an increase in cardiomyocyte proliferation. Those cardiomyocytes positive for thymidine analog labeling were characteristic of newly generated cardiomyocytes, with smaller size and single diploid nuclei. Moreover, newly generated cardiomyocytes were found to reside in close proximity to one another, consistent with regional stimulation of cellular proliferation.
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