Configuration of bacteriological evidence of causative pathogens in patients with aspiration pneumonia using the bacterial floral analysis of 16S ribosomal RNA gene

• Project/Area Number: 26461172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: Mukae Hiroshi 産業医科大学, 医学部, 非常勤医師 (80253821)
• Research Collaborator: AKATA Kentaro ; KAWANAMI Toshinori ; NOGUCHI Shingo ; NAITO Keisuke ; HATA Ryosuke ; NISHIDA Chinatsu ; YAMASAKI Kei ; KIDO Takashi ; YATERA Kazuhiro
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 誤嚥性肺炎 / 口腔レンサ球菌 / 嫌気性菌 / 細菌叢解析 / 16S rRNA / 口腔内衛生状態 / 連鎖球菌 / 16s rRNA

Outline of Final Research Achievements

We evaluated causative bacterial species by the clone library method using 16S rRNA gene sequencing analysis of bronchoalveolar lavage fluid in pneumonia patients (n=177) with or without aspiration risks. Oral streptococci were significantly detected in patients with aspiration risks (n=83) compared with those without, and factors such as poor ECOG performance status, past history of pneumonia within one year were associated with a detection of oral streptococci. These results suggest that oral streptococci are more important as causative pathogens of pneumonia in patients with aspiration pneumonia than previously believed. In addition, studies evaluated the relationship between oral hygiene status and detected bacterial species showed that poor oral hygiene status was significantly related to higher detection of anaerobic bacteria, and therefore oral hygiene status might be important factor for detecting anaerobic bacteria in the lower respiratory tract infection.

Research Products

• [Journal Article] Determining the possible etiology of hospital acquires pneumonia using a clone library analysis in Japan (2017)
  • Author(s): Yatera K, Noguchi S, Yamasaki K, Kawanami T, Fukuda K, Naito K, Akata K, Kido T, Ishimoto H, Sakamoto N, Taniguchi H, Mukae H
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 印刷中
  • NAID: 130006776923
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Significance of Oral Streptococci in Patients with Pneumonia with Risk Factors for Aspiration: The Bacterial Floral Analysis of 16S Ribosomal RNA Gene using Bronchoalveolar Lavage Fluid. (2016)
  • Author(s): Akata K, Yatera K, Yamasaki K, Kawanami T, Naito K, Noguchi S, Fukuda K, Ishimoto H, Taniguchi H, Mukae H
  • Journal Title: BMC Pulmonary Medicine Volume: 16 Issue: 1 Pages: 1-12
  • DOI: 10.1186/s12890-016-0235-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinical impact of methicillin-resistant Staphylococcus aureus on bacterial pneumonia: cultivation and 16S ribosomal RNA gene analysis of bronchoalveolar lavage fluid (2016)
  • Author(s): Kawanami T, Yatera K, Yamasaki K, Noguchi S, Fukuda K, Naito K, Akata K, Akata K, Kido T, Ishimoto H, Taniguchi H, Mukae H.
  • Journal Title: BMC Infect Dis Volume: 15 Issue: 1 Pages: 155-155
  • DOI: 10.1186/s12879-016-1493-3
  • Peer Reviewed / Open Access
• [Journal Article] The importanceof obligate anaerobes and the streptococcus anginosus group in pulmonary abscess a clone library analysis using bronchoalveolar lavage fluid (2016)
  • Author(s): Mukae H, Noguchi S, Naito K, Kawanami T, Yamasaki K, Fukuda K, Akata K, Fukuda Y, Kido T, Ishimoto H, Sakamoto N, Taniguchi H, Yatera K
  • Journal Title: Respiration Volume: 92 Issue: 2 Pages: 80-89
  • DOI: 10.1159/000447976
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 【日常よく見る呼吸器感染症】誤嚥性肺炎の病態と臨床 (2016)
  • Author(s): 矢寺和博，迎 寛
  • Journal Title: 呼吸器内科 Volume: 28 Pages: 39-44
• [Journal Article] 特集「肺炎の現状と管理 ２．原因菌の変遷ー最新技術を用いた解析ー (2016)
  • Author(s): 川波敏則，迎 寛
  • Journal Title: 化学療法の領域 Volume: 32 Pages: 32-38
• [Journal Article] Ｂａｃｔｅｒｉｏｌｏｇｉｃａｌ ａｓｓｅｓｓｍｅｎｔ ｏｆ ｈｅａｌｔｈｃａｒｅ ａｓｓｏｃｉａｔｅｄ ｐｎｅｕｍｏｎｉａ ｕｓｉｎｇ ａ ｃｌｏｎｅ ｌｉｂｒａｒｙ ａｎａｌｙｓｉｓ (2015)
  • Author(s): Ｎｏｇｕｃｈｉ Ｓ， Ｍｕｋａｅ Ｈ，Kawanami T, Yamasaki K, Fukuda K, Akata K, Ishimoto H, Taniguchi H, Yatera K,
  • Journal Title: ＰＬＯＳ ＯＮＥ Volume: 印刷中 Issue: 4 Pages: 10-10
  • DOI: 10.1371/journal.pone.0124697
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ｐｏｓｓｉｂｌｅ ｒｏｌｅ ｏｆ ａｎａｅｒｏｂｅｓ ｉｎ ｔｈｅ ｐａｔｈｏｇｅｎｅｓｉｓ ｏｆ ｎｏｎｔｕｂｅｒｃｕｌｏｓｉｓ ｍｙｃｏｂａｃｔｅｒｉａｌ ｉｎｆｅｃｔｉｏｎ (2015)
  • Author(s): Ｙａｍａｓａｋｉ Ｋ, Mukae H, Kawanami T, Fukuda K, Noguchi S, Akata K, Naito K, Oda K, Ogoshi T, Nishida C, Orihashi T, Kawanami Y, Ishimoto H, Taniguchi H, Yatera K,
  • Journal Title: Ｒｅｓｐｉｒｏｌｏｇｙ Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] An unclassified microorganism: novel pathogen candidate lurking in human airways (2014)
  • Author(s): Fukuda K, Yatera K, Ogawa M, Kawanami T, Yamasaki K, Noguchi S, Murphy RS, Mukae H, Taniguchi H,
  • Journal Title: PLOS ONE Volume: 31 Issue: 7 Pages: e103646-e103646
  • DOI: 10.1371/journal.pone.0103646
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 細菌叢解析法用いた細菌性肺炎における潜在性耐性菌の臨床的背景および危険因子の解析 (2016)
  • Author(s): 川波敏則，野口真吾，赤田憲太朗，内藤圭祐，高木努，小田桂士，山崎啓，城戸貴志，迎 寛，矢寺和博
  • Organizer: 第86回日本感染症学会西日本地方会学術集会・第64回日本化学療法学会西日本支部総会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県那覇市)
  • Year and Date: 2016-11-24
• [Presentation] Clinical Impact of Methicillin-Resistant Staphylococcus Aureus on Bacteria Pneumonia: Comparison of the Results of Cultivation and the Molecular Method of 16S Ribosomal RNA Gene Analysis Using Bronchoalveolar Lavage Fluid (2016)
  • Author(s): Kawanami T,Yatera K, Yamasaki K, Noguchi S, Fukuda K, Akata K, Naito K, Kido T, Ishimoto H, Taniguchi H, Mukae H
  • Organizer: American Thorasic Society International Conference 2016
  • Place of Presentation: Moscone Center(San Francisco, USA)
  • Year and Date: 2016-05-13
  • Int'l Joint Research
• [Presentation] 細菌叢解析法を用いた細菌性肺炎における嫌気性菌の臨床的背景の検討 (2016)
  • Author(s): 川波敏則，赤田憲太朗，山崎啓，野口真吾，内藤圭祐，城戸貴志，石本裕士，矢寺和博，迎 寛
  • Organizer: 第90回日本感染症学会総会・学術講演会
  • Place of Presentation: 仙台国際センター・新展示施設 （宮城県仙台市）
  • Year and Date: 2016-04-15
• [Presentation] 16S ribosomal RNA 遺伝子を用いた肺化膿症の細菌叢解析の検討 (2016)
  • Author(s): 内藤圭祐，野口真吾，赤田憲太朗，山崎啓，川波敏則，花香未奈子，生越貴明，西田千夏，城戸貴志，石本裕士，矢寺和博，迎 寛
  • Organizer: 第56回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2016-04-08
• [Presentation] Streptococcus anginosus groupによる呼吸器感染症の臨床的特徴の検討 (2016)
  • Author(s): 野口真吾，矢寺和博，川波敏則，山崎啓，内藤圭祐，赤田憲太朗，島袋活子，石本裕士，吉井千春，迎 寛
  • Organizer: 第56回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2016-04-08
• [Presentation] 網羅的細菌叢解析からみた呼吸器感染症の原因菌（シンポジウム） (2016)
  • Author(s): 野口真吾，矢寺和博，川波敏則，山崎啓，迎 寛
  • Organizer: 第56回日本呼吸器学会学術講演会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
  • Year and Date: 2016-04-08
• [Presentation] 成人肺炎における嫌気性菌関与を予測する因子の検討～網羅的細菌叢解析を用いて～ (2016)
  • Author(s): 赤田憲太朗, 矢寺和博, 川波敏則, 内藤圭祐, 野口真吾, 山崎啓, 迎 寛
  • Organizer: 第46回日本嫌気性菌感染症学会総会
  • Place of Presentation: 長崎大学医学部良順会館 (長崎県長崎市)
  • Year and Date: 2016-03-04
• [Presentation] 呼吸器感染症領域における嫌気性菌の重要性 (2016)
  • Author(s): 迎 寛
  • Organizer: 第46回日本嫌気性菌感染症学会総会
  • Place of Presentation: 長崎大学医学部良順会館 (長崎県長崎市)
  • Year and Date: 2016-03-04
  • Invited
• [Presentation] "Bacteriological etiology in pneumonia patients with pulmonary emphysema using (2016)
  • Author(s): Naito K, Yamasaki K, Yatera K, Kawanami T, Akata K, Kido T, Ishimoto H, Aoki T,Mukae H
  • Organizer: Asia Oceania Workshop of Pulmonary Functional Imaging
  • Place of Presentation: 兵庫県立 淡路夢舞台国際会議場 (兵庫県淡路市)
  • Year and Date: 2016-01-29
  • Int'l Joint Research
• [Presentation] 肺炎診療：細菌叢解析で分かった新たな知見～呼吸器感染症における嫌気性菌の役割 (2015)
  • Author(s): 迎 寛
  • Organizer: 第85回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 奈良春日野国際フォーラム 甍 (奈良県奈良市)
  • Year and Date: 2015-10-15
  • Invited
• [Presentation] 細菌叢解析法を用いた細菌性肺炎におけるMRSAの臨床的意義の解析 (2015)
  • Author(s): 川波敏則, 野口真吾, 山崎 啓, 赤田憲太朗, 内藤圭祐, 石本裕士, 矢寺和博, 迎 寛
  • Organizer: 第85回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 奈良春日野国際フォーラム 甍 (奈良県奈良市)
  • Year and Date: 2015-10-15
• [Presentation] 肺気腫における画像所見と細菌学的所見の臨床的検討 (2015)
  • Author(s): 山崎 啓, 矢寺和博, 赤田憲太朗, 内藤圭祐, 野口真吾, 川波敏則, 西田千夏, 川波由紀子, 石本裕士, 迎 寛
  • Organizer: 第85回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 奈良春日野国際フォーラム 甍 (奈良県奈良市)
  • Year and Date: 2015-10-15
• [Presentation] Bacteriological assessment of healthcare assosciated pnumonia using aclone library analysis (2015)
  • Author(s): 野口真吾, 矢寺和博, 川波敏則, 山崎 啓, 福田和正, 赤田憲太朗, 西田千夏, 石本裕士, 谷口初美, 迎 寛
  • Organizer: 第75回日本呼吸器学会九州支部秋季学術講演会
  • Place of Presentation: ホテルグランデはがくれ (佐賀県佐賀市)
  • Year and Date: 2015-10-02
• [Presentation] The bacteriological incidence of lung abscess using clone library analysis of 16S rRNA gene in bronchoalveolar lavage fluid (2015)
  • Author(s): Naito K, Noguchi S, Yatera K, Akata K, Nishida C, Yamasaki K, Orihashi T, Kawanami T,Ishimoto H,Fukuda K, Taniguchi H,Mukae H
  • Organizer: European respiratory society International congress 2015
  • Place of Presentation: Amsterdam (the Netherlands)
  • Year and Date: 2015-09-26
  • Int'l Joint Research
• [Presentation] Bacteriological assessment of bronchoalveolar lavage fluid using clone library analysis of 16S rRNA gene revealed a significance of oral streptococci in patients with aspiration pneumonia. (2015)
  • Author(s): Akata K, Yatera K, Yamasaki K, Kawanami T, Naito K, Noguchi S, Fukuda K,Ishimoto H,Taniguchi H, Mukae H
  • Organizer: American Thorasic Society 2015 International Conference
  • Place of Presentation: Denver (USA)
  • Year and Date: 2015-05-15
  • Int'l Joint Research
• [Presentation] 細菌叢解析からみた肺炎における口腔内レンサ球菌の重要性 (2015)
  • Author(s): 赤田憲太朗, 川波敏則, 矢寺和博,内藤圭祐, 野口真吾, 山崎 啓, 西田千夏, 渡橋 剛, 川波由紀子, 石本裕士, 迎 寛
  • Organizer: 第89回日本感染症学会総会・学術講演
  • Place of Presentation: 国立京都国際会館 (京都府京都市)
  • Year and Date: 2015-04-16
• [Presentation] 細菌叢解析を用いたい院内肺炎(Hospital-Acquired Pneumonia;HAP)の原因菌の検討 (2015)
  • Author(s): 矢寺和博, 野口真吾, 川波敏則, 山崎 啓, 内藤圭祐, 赤田憲太朗, 石本裕士, 福田和正,谷口初美, 迎 寛
  • Organizer: 第112回日本内科学会講演会
  • Place of Presentation: みやこめっせ (京都府京都市)
  • Year and Date: 2015-04-10
• [Presentation] 16S ribosomal RNA遺伝子検索を用いてnocardia exalbidaを推定しえた肺ノカルジア症の1例 (2014)
  • Author(s): 内藤圭祐, 赤田憲太朗, 野口真吾, 山﨑啓, 西田千夏, 渡橋剛, 川波敏則, 川波由紀子, 石本裕士, 矢寺和博, 迎 寛,
  • Organizer: 第84回感染症学会西日本地方会
  • Place of Presentation: 岡山コンベンションセンター(岡山)
  • Year and Date: 2014-10-23 – 2014-10-25
• [Presentation] Roles of anaerobes in the pathogenesis of notuberculosis mycobacterial infection (2014)
  • Author(s): Yamasaki K, Mukae H, Kawanami T, Fukuda K, Noguchi S, Akata K, Naito K, Oda K, Ogoshi T, NIshida C, Orihashi T, Kawanami Y, Ishimoto H, Taniguchi H, Yatera K,
  • Organizer: European respiratory society 2014 international congress
  • Place of Presentation: Munich(Germany)
  • Year and Date: 2014-09-06 – 2014-09-10
• [Presentation] 肺炎ガイドラインに沿った抗菌薬の使い分け (2014)
  • Author(s): 迎 寛
  • Organizer: 第51回日本呼吸器学会中国四国地方会
  • Place of Presentation: 米子コンベンションセンター(鳥取)
  • Year and Date: 2014-07-11 – 2014-07-12
  • Invited
• [Presentation] ガイドラインを用いた肺炎の診断と治療 (2014)
  • Author(s): 迎 寛
  • Organizer: 第89回日本結核病学会総会
  • Place of Presentation: 長良川国際会議場(岐阜)
  • Year and Date: 2014-05-09 – 2014-05-10
  • Invited
• [Presentation] 呼吸器感染症の治療戦略 (2014)
  • Author(s): 迎 寛
  • Organizer: 第54回日本呼吸器学会学術講演会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2014-04-25 – 2014-04-27
  • Invited
• [Book] 呼吸器疾患 最新の治療2016-2018 (2016)
  • Author(s): 川波敏則，迎 寛
  • Total Pages: 494
  • Publisher: 南江堂
• [Book] 抗菌薬パーフェクトガイド (2016)
  • Author(s): 野口真吾，迎 寛
  • Total Pages: 359
  • Publisher: ヴァンメディカル
• [Book] 分子呼吸器学 (2015)
  • Author(s): 野口真吾, 迎 寛
  • Total Pages: 3
  • Publisher: 先端医学社
• [Book] 肺炎の診断 遺伝子診断 (2015)
  • Author(s): 赤田憲太朗, 迎 寛
  • Total Pages: 5
  • Publisher: 大道学館