Biomarker study for multiple sclerosis with Imaging Mass Spectrometry

• Project/Area Number: 26461300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Doshisha University
• Principal Investigator: Ikegawa Masaya 同志社大学, 生命医科学部, 教授 (60381943)
• Co-Investigator(Kenkyū-buntansha): 近藤 誉之 京都大学, 医学研究科, 准教授 (50322756)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 多発性硬化症 / バイオマーカー / イメージング質量分析法 / プロテオミクス / 多変量解析 / EAE / 実験的自己免疫性脳脊髄炎 / EAEマウス / イメージングマススペクトロメトリー / メタボロミクス

Outline of Final Research Achievements

Experimental autoimmune encephalomyelitis (EAE) is the most commonly used experimental model for the human inflammatory demyelinating disease, multiple sclerosis (MS). In this study, we applied matrix-assisted laser desorption/ionization (MALDI) mass imaging mass spectrometry (IMS) for EAE mice brain and spinal cord.Here we systematically visualized the distribution of proteins and peptides from brains and spinal cords of the mice during EAE development. Using multivariate analysis, area specific proteomic pattern was extracted in wild type mice and aberrant distribution was noticed in EAE mice even from early to onset stage of the diseases. For the clarification of pathogenesis of MS, we consider proteomic IMS on brain and spinal cord sections from EAE mice is a valuable approach.

Research Products

• [Presentation] Imaging Mass Spectrometry (IMS) for the proteomic studies of EAE mouse (2016)
  • Author(s): Hiroki Yamashita, Noriyuki Iwasaki, Takashi Nirasawa, Nobuto Kakuda, Yuki Nakagawa, Katsutoshi Taguchi, Masaki Tanaka, Takayuki Kondo, Masaya Ikegawa
  • Organizer: HUPO
  • Place of Presentation: 台北、中華民国
  • Year and Date: 2016-09-18
  • Int'l Joint Research
• [Presentation] Proteomic Imaging for Brain and Spinal Cord from Experimental Allergic Encephalomyelitis (EAE) Mouse Model (2015)
  • Author(s): Takashi Nirasawa, Noriyuki Iwasaki, Takayuki Kondo, Akimitsu Miyake, Hiroki Yamashita, Masaya Ikegawa
  • Organizer: 第63回アメリカ質量分析学会
  • Place of Presentation: アメリカ セントルイス
  • Year and Date: 2015-05-31 – 2015-06-04
• [Presentation] Proteomic Imaging for Brain and Spinal Cord from Experimental Allergic Encephalomyelitis (EAE) Mouse Model (2015)
  • Author(s): Takashi Nirasawa, Noriyuki Iwasaki, Takayuki Kondo, Akimitsu Miyake, Hiroki Yamashita, Masaya Ikegawa
  • Organizer: American Society for Mass Spectrometry
  • Place of Presentation: St. Louis, UA
  • Year and Date: 2015-05-31
  • Int'l Joint Research