Genome wide methylation analysis for homeostasis of pancreatic beta-cells
| Project/Area Number |
26461351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | DNAメチル化 / 糖尿病 / 遺伝子 / 発現制御 / 細胞・組織 / 膵β細胞 / 内分泌 / 転写因子 |
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| Outline of Final Research Achievements |
Relationship between cell function, gene expression and DNA methylation was analyzed in pancreatic beta-cells. In the terminal differentiation process for maturation of pancreatic islets, genome-wide inhibition in gene expression was observed, in which DNA methylation was involved. These genes were downregulated in FACS-sorted mature beta-cells but upregulated in the islets of diabetes model mice. Demethylation of promoter of Mafb, one of these genes downregulated in mature beta-cells, was observed with increased promoter activity and gene expression in glucotoxic beta-cell model. These results suggested that DNA methylation is involved in gene expression and function of beta-cells in the process of terminal differentiation and deterioration in diabetes.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Usefulness of miRNA profiles for predicting pathological responses to neoadjuvant chemotherapy in patients with human epidermal growth factor receptor 2-positive breast cancer.2017
Author(s)
Ohzawa H, Miki A, Teratani T, Shiba S, Sakura Y, Nishimura W, Noda Y, Fukushima N, Fuji H, Hozumi Y, Mukai H, Yasuda Y.
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Journal Title
Oncology Letters
Volume: 13巻
Issue: 3
Pages: 1731-1740
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Paternal allelic mutation at the Kcnq1 locus reduces pancreatic β cell mass via epigenetic modification of Cdkn1c.2015
Author(s)
Asahara S, Etoh H, Inoue H, Teruyama K, Shibutani Y, Ihara Y, Kawada Y, Bartolome A, Hashimoto N, Matsuda T, Koyanagi-Kimura M, Kanno A, Hirota Y, Hosooka T, Nagashima K, Nishimura W, Inoue H, Matsumoto M, Higgins MJ, Yasuda K, Inagaki N, Seino S, Kasuga M, Kido Y.
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Journal Title
Proceedings of the National Acaddemy of Scienceof the United States of America
Volume: 112(27)
Issue: 27
Pages: 8332-8337
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comparison between the antiemetic effects of palonosetron and granisetron in breast cancer patients treated with anthracycline-based regimens.2015
Author(s)
Ohzawa H, Miki A, Hozumi Y, Miyazaki C, Sagara Y, Tanaka Y, Shiba S, Joutoku H, Sakuragi M, Takehara M, Sakuma Y, Nishimura W, Fujii H, Yasuda Y.
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Journal Title
Oncology Letters
Volume: 9
Issue: 1
Pages: 119-124
DOI
Related Report
Peer Reviewed
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