| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Neutrophils contribute to antimicrobial defense by releasing nuclear chromatin together with granule proteins to form an extra cellular mesh which binds and kills bacteria.This formation, called neutrophil extracellular traps (NETs), considered to be implicated in anti-nucleotide antibody (ANA) positive Systemic Lupus Erythematosus (SLE).Activated neutrophils has been shown to involve the NADPH oxidase (NOX2) mediated production of reactive oxygen species (ROS), leading to disintegration of the nuclear envelope and result in induction of NETosis. In this study, we investigated the causal relation between NOX2 dependent/independent ROS production and extracellular DNA releasing. It was observed that the deletion of NOX2 in neutrophils accelerated mitochondrial ROS production with lps stimulation and released oxidative damaged DNA. Addition of anti-oxDNA antibodies induced more NETosis in vitro assay. Therefore, further analysis of the pathogenesis of anti-oxDNA Ab is needed.
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