Integrated genomic analysis of aggressive subtype of neuroblastoma
Project/Area Number |
26461603
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
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Research Institution | Research Institute for Clinical Oncology, Saitama Cancer Center (2015-2016) Chiba Cancer Center (Research Institute) (2014) |
Principal Investigator |
Ohira Miki 埼玉県立がんセンター(臨床腫瘍研究所), 臨床腫瘍研究所, 主幹研究員 (20311384)
|
Co-Investigator(Renkei-kenkyūsha) |
NAKAGAWARA Akira 佐賀県医療センター好生館, 理事長 (50117181)
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 神経芽腫 / ゲノム / 遺伝子 / マイクロアレイ / 癌 |
Outline of Final Research Achievements |
Neuroblastoma (NB) is the most common pediatric extracranial solid tumor, originating from the sympathoadrenal lineage of neural crest. Despite intense multi-modal therapy, NB remains the second most common cause of cancer deaths among children. To elucidate molecular basis for malignant type of NB, we have conducted integrated genomic analyses, including array CGH, whole exome sequencing, transcriptome and methylome profiling. Whole exome sequencing of 30 high stage primary tumors identified approximately 20 non-silent somatic mutations and indels per tumor in average, among which 27 were recurrent mutations appeared >2. Pathway analysis revealed enrichment of the mutated genes in the MAPK pathway and the axon guidance pathway. Methylome profiling suggested the existence of three clusters with different survival rates. Altogether, these genomic features can help understand NB pathogenesis and contribute risk stratification for therapy.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Genomic analysis-integrated whole-exome sequencing of neuroblastoma identifies genetic mutations in axon guidance pathway.2017
Author(s)
Li Y*, Ohira M*, Zhou Y, Xiong T, luo W, Yang C, Li X, Gao Z, Nakamura Y, Kamijo T, Kaneko Y, Taketani T, Ueyama J, Tajiri T, Zhang H, Wang J, Yang H, Yin Y, Nakagawara A.
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Journal Title
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Neuronal leucine-rich repeat 1 negatively regulates anaplastic lymphoma kinase in neuroblastoma.2016
Author(s)
Satoh S, Takatori A, Ogura A, Kohashi K, Souzaki R, Kinoshita Y, Taguchi T, Hossain MS, Ohira M, Nakamura Y, Nakagawara A.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 32682-32682
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Revised risk estimation and treatment stratification of low- and intermediate-risk neuroblastoma patients by integrating clinical and molecular prognostic markers.2015
Author(s)
Oberthuer A, Juraeva D, Hero B, Volland R, Carolina S, Schmidt R, Faldum A, Kahlert Y, Engesser A, Asgharzadeh S, Seeger RC, Ohira M, Nakagawara A, Scaruffi P, at all
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Journal Title
Clin Cancer Res.
Volume: 21(8)
Issue: 8
Pages: 1904-1915
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Oncogenic <i>Lmo3</i> cooperates with <i>Hen2</i> to induce hydrocephalus in mice2015
Author(s)
Isogai E, Okumura K ,Saito M, Yoshizawa Y, Itoh K, Tando S, Ohira M, Haraguchi S, Nakagawara A, Fushiki S, Nagase H, Wakabayashi Y
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Journal Title
Experimental Animals
Volume: 64
Issue: 4
Pages: 407-414
DOI
NAID
ISSN
0007-5124, 1341-1357, 1881-7122
Related Report
Peer Reviewed / Open Access
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[Journal Article] Flotillin-1 regulates oncogenic signaling in neuroblastoma cells by regulating ALK membrane association.2014
Author(s)
Tomiyama A, Uekita T, Kamata R, Sasaki K, Takita J, Ohira M, Nakagawara A, Kitanaka C, Mori K, Yamaguchi H, Sakai R.
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Journal Title
Cancer Res.
Volume: 74(14)
Issue: 14
Pages: 3790-3801
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Clinical relevance of genomic and epigenomic classification of MYCN-non-amplified neuroblastoma2016
Author(s)
Ohira M, Nakamura Y, Tatsumi Y, Tatsuno K, Tsutsumi S, Yamamoto S, Nagae G, Renard-Guillet C, Nagase H, Kamijo T, Aburatani H, Nakagawara A
Organizer
ANR2016
Place of Presentation
ケアンズ(オーストラリア)
Year and Date
2016-06-19
Related Report
Int'l Joint Research
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[Presentation] Genomic characterization of high-risk neuroblastoma in Japan: A retrospective study of 537 cases by using updated follow-up data based on INRG variables [JNBSG (Japan Neuroblastoma Study Group)]2016
Author(s)
Ohira M, Kamijo T, Takimoto T, Nakazawa A, Matsumoto K, Shichino H, Hishiki T, Iehara T, Nakamura Y, Nagase H, Yoneda A, Fukushima T, Tajiri T, Nakagawara A
Organizer
ANR2016
Place of Presentation
ケアンズ( オーストラリア)
Year and Date
2016-06-19
Related Report
Int'l Joint Research
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[Presentation] Genomic landscape of aggressive tumor subtype in MYCN-non-amplified neuroblastoma2016
Author(s)
Ohira M, Tatsuno K, Nakamura Y, Tatsumi Y, Tsutsumi S, Yamamoto S, Nagae G, Renard-Guillet C, Nagase H, Kamijo T, Aburatani H, Nakagawara A
Organizer
AACR Annual Meeting 2016
Place of Presentation
ニューオーリンズ(米国)
Year and Date
2016-04-16
Related Report
Int'l Joint Research
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[Presentation] Genomic profiling of intermediate risk type of neuroblastoma without MYCN amplification.2015
Author(s)
M.Ohira, Y.Nakamura, K.Tatsuno, S.Tsutsumi, S.Yamamoto, G.Nagae, Y.Tatsumi, H.Nagase, T.Kamijo, H.Aburatani, A.Nakagawara.
Organizer
第57回日本小児血液・がん学会学術集会
Place of Presentation
甲府市
Year and Date
2015-11-27
Related Report
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[Presentation] Genomic characterization of aggressive subtypes of neuroblastoma with 11q partial loss.2015
Author(s)
M.Ohira, Y.Nakamura, K.Tatsuno, S.Tsutsumi, S.Yamamoto, G.Nagae, Y.Tatsumi, H.Nagase, T.Kamijo, H.Aburatani, A.Nakagawara.
Organizer
神経芽腫アジアパシフィックシンポジウム2015
Place of Presentation
台湾・台北市
Year and Date
2015-11-13
Related Report
Int'l Joint Research
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[Presentation] 11q欠失を伴った難治性中間リスク型神経芽腫のゲノム・エピゲノム解析.2015
Author(s)
大平美紀, 中村洋子, 辰野健二, 堤修一, 山本尚吾, 永江玄太, 巽康年, 永瀬浩喜,上條岳彦, 油谷浩幸, 中川原章.
Organizer
第74回日本癌学会学術集会
Place of Presentation
名古屋市
Year and Date
2015-10-08
Related Report
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