Pathogenic microorganism identification in neonatal infectious diseases by a meta-genomic analysis using the next generation sequencer
Project/Area Number |
26461632
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Embryonic/Neonatal medicine
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Research Institution | Kobe University |
Principal Investigator |
MORIOKA Ichiro 神戸大学, 医学研究科, 特命教授 (80437467)
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Co-Investigator(Kenkyū-buntansha) |
池田 真理子 (谷口 / 池田 真理子(谷口真理子)) 神戸大学, 医学研究科, 特命准教授 (00410738)
竹島 泰弘 神戸大学, 医学研究科, 客員教授 (40281141)
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Co-Investigator(Renkei-kenkyūsha) |
MORI Yasuko 神戸大学, 大学院医学研究科, 教授 (50343257)
DEGUCHI Masashi 神戸大学, 大学院医学研究科, 特命教授 (50403291)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 次世代シークエンサー / メタゲノミック診断法 / 新生児感染症 / 小児感染症 / 周産期・新生児感染症 / 胎盤 / 臍帯 |
Outline of Final Research Achievements |
The purpose of this study was to establish a meta-genomic analysis using the next generation sequencer for perinatal and neonatal infectious diseases, and to identify pathogenic microorganisms that had been difficult to identify to date. As results, a meta-genomic analysis system using the next generation sequencer was established during the study period and we applied to the neonatal and pediatric patients with infection. We obtained new findings from patient specimens using these techniques, and for the first time, reported the cases to the international journals, such as a newborn with persistent pulmonary hypertension who was detected echovirus 7 from not only the serum, nasal swab and bronchoalveolar lavage, but also the preserved umbilical cord, a pediatric patient due to enterovirus D68 infection that developed interstitial pneumonia, and a newborn with congenital cytomegalovirus infection with retinal arterio-venous anastomosis.
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Report
(4 results)
Research Products
(132 results)
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[Journal Article] Changes in the numbers of patients with acute gastroenteritis after voluntary introduction of the rotavirus vaccine in a Japanese children’s primary emergency medical center.2017
Author(s)
Morioka I, Kamiyoshi N, Nishiyama M, Yamamura T, Minamikawa S, Iwatani S, Nagase H, Nozu K, Nishimura N, Taniguchi-Ikeda M, Ishibashi K, Ishida A, Iijima K
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Journal Title
Environ Health Prev Med.
Volume: 22
Issue: 1
Pages: 15-15
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Novel missense mutation in DLL4 in a Japanese sporadic case of Adams-Oliver syndrome.2017
Author(s)
Nagasaka M, Taniguchi-Ikeda M, Inagaki H, Ouchi Y, Kuroda D, Yamana K, Harada R, Nozu K, Sakai Y, Morikoka I, Toda T, Kurahashi H, Iijima K
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A case of harlequin ichthyosis with a favorable outcome: early treatment and novel, differentially expressed, alternatively spliced transcripts of the ATP-binding cassette sub-family A member 12 gene.2017
Author(s)
Washio K, Sumi M, Nakata K, Fukunaga A, Yamana K, Koda T, Morioka I, Nishigori C, Yamanishi K
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Characterization of contiguous gene deletions in COL4A6 and COL4A5 in Alport syndrome-diffuse leiomyomatosis.2017
Author(s)
Nozu K, Minamikawa S, Yamada S, Oka M, Yanagita M, Morisada N, Fujinaga S, Nagano C, Gotoh Y, Takahashi E, Morishita T, Yamamura T, Ninchoji T, Kaito H, Morioka I, Nakanishi K, Vorechovsky I, Iijima K
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Journal Title
J Hum Genet.
Volume: Epub 2017 Mar 9.
Issue: 7
Pages: 733-735
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A birth of bipartite exon by intragenic deletion.2017
Author(s)
Nozu K, Iijima K, Igarashi T, Yamada S, Kralovicova J, Nozu Y, Yamamura T, Minamikawa S, Morioka I, Ninchoji T, Kaito H, Nakanishi K, Vorechovsky I
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Journal Title
Mol Genet Genomic Med.
Volume: 印刷中
Issue: 3
Pages: 287-294
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Cryptic exon activation in SLC12A3 in Gitelman syndrome.2017
Author(s)
Nozu K, Nozu Y, Nakanishi K, Konomoto T, Horinouchi T, Shono A, Morisada N, Minamikawa S, Yamamura T, Fujimura J, Nakanishi K, Ninchoji T, Kaito H, Morioka I, Taniguchi-Ikeda M, Vorechovsky I, Iijima K
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Journal Title
J Hum Genet.
Volume: 62
Issue: 2
Pages: 335-337
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] A pediatric patient with interstitial pneumonia due to enterovirus D68.2016
Author(s)
Matsumoto M, Awano H, Ogi M, Tomioka K, Unzaki A, Nishiyama M, Toyoshima D, Taniguchi-Ikeda M, Ishida A, Nagase H, Morioka I, Iijima K
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Journal Title
J Infect Chemother.
Volume: 22
Issue: 10
Pages: 712-715
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Rare renal ciliopathies in non-consanguineous families that were identified by targeted resequencing.2016
Author(s)
Yamamura T, Morisada N, Nozu K, Minamikawa S, Ishimori S, Toyoshima D, Ninchoji T, Yasui M, Taniguchi-Ikeda M, Morioka I, Nakanishi K, Nishio H, Iijima K.
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Journal Title
Clin Exp Nephrol.
Volume: 20
Issue: 1
Pages: 136-142
DOI
Related Report
Peer Reviewed
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[Journal Article] Next-generation sequencing discloses a nonsense mutation in the dystrophin gene from long preserved dried umbilical cord and low-level somatic mosaicism in the proband mother.2016
Author(s)
Taniguchi-Ikeda M, Takeshima Y, Lee T, Nishiyama M, Awano H, Yagi M, Unzaki A, Nozu K, Nishio H, Matsuo M, Kurahashi H, Toda T, Morioka I, Iijima K.
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Journal Title
J Hum Genet.
Volume: 61
Issue: 4
Pages: 351-355
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Neurological outcomes in symptomatic congenital cytomegalovirus-infected infants after introduction of newborn urine screening and antiviral treatment.2016
Author(s)
Nishida K, Morioka I, Nakamachi Y, Kobayashi Y, Imanishi T, Kawano S, Iwatani S, Koda T, Deguchi M, Tanimura K, Yamashita D, Nibu K, Funakoshi T, Ohashi M, Inoue N, Iijima K, Yamada H
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Journal Title
Brain Dev.
Volume: 38
Issue: 2
Pages: 209-216
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] A novel PIGN mutation and prenatal diagnosis of inherited glycosylphosphatidylinositol deficiency.2015
Author(s)
Nakagawa T, Taniguchi-Ikeda M, Murakami Y, Nakamura S, Motooka D, Emoto T, Satake W, Nishiyama M, Toyoshima D, Morisada N, Takada S, Tairaku S, Okamoto N, Morioka I, Kurahashi H, Toda T, Kinoshita T, Iijima K.
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Journal Title
American Journal of Medical Genetics PartA
Volume: 170
Issue: 1
Pages: 183-188
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Low total IgM values and high cytomegalovirus loads in the blood of newborns with symptomatic congenital cytomegalovirus infection.2015
Author(s)
Kobayashi Y, Morioka I, Koda T, Nakamachi Y, Okazaki Y, Noguchi Y, Ogi M, Chikahira M, Tanimura K, Ebina Y, Funakoshi T, Ohashi M, Iijima K, Inoue N, Kawano S, Yamada H
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Journal Title
J Perinatal Med
Volume: 43
Issue: 2
Pages: 239-243
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] Changes in patients with acute gastroenteritis after voluntary introduction of rotavirus vaccine in a Japanese children’s primary emergency medical center.2016
Author(s)
Kamiyoshi N, Iwatani S, Nishiyama M, Yamamura T, Minamikawa S, Taniguchi-Ikeda M, Ishibashi K, Iijima K, Ishida A, Morioka I
Organizer
12nd Congress of Asian Society for Pediatric Research
Place of Presentation
Bangkok, Thailand
Year and Date
2016-11-10
Related Report
Int'l Joint Research
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[Presentation] A pediatric patient with interstitial pneumonia due to enterovirus D68.2016
Author(s)
Matsumoto M, Awano H, Ogi M, Tomioka K, Unzaki A, Nishiyama M, Toyoshima D, Taniguchi-Ikeda M, Ishida A, Nagase H, Morioka I, Iijima K
Organizer
12nd Congress of Asian Society for Pediatric Research
Place of Presentation
Bangkok, Thailand
Year and Date
2016-11-10
Related Report
Int'l Joint Research
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[Presentation] Countermeasures against congenital toxoplasmosis and cytomegalovirus infection led by the research team under the supervision of the Ministry of Health, Labor and Welfare in Japan.2016
Author(s)
Fujii T, Ikeda T, Inoue N, Kanayama N, Kawana T, Kimura H, Kobayashi Y, Koyano S, Masuzaki H, Matsuyama H, Minematsu T, Morioka I, Moriuchi H, Oka A, Saito S, Samejima H, Suzutani T, Yamada H, Yoshikawa T
Organizer
CMV Public Health & Policy Conference
Place of Presentation
Austin, Texas, USA
Year and Date
2016-09-26
Related Report
Int'l Joint Research
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[Presentation] Advantage of next generation sequencing in molecular diagnosis in DMD -mutation screening with long preserved dried umbilical cord and detection of mosaicism-.2016
Author(s)
Taniguchi-Ikeda M, Unzaki A, Takeshima Y, Lee T, Awano H, Yagi M, Kurahashi H, Morioka I, Toda T, Matsuo M, Iijima K
Organizer
13th International Congress of Human Genetics
Place of Presentation
Kyoto, Japan
Year and Date
2016-04-03
Related Report
Int'l Joint Research
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[Presentation] Advantage of next generation sequencing in molecular diagnosis in DMD - mutation screening with long preserved dried umbilical cord and detection of mosaicism-2016
Author(s)
Unzaki A, Taniguchi-Ikeda M, Takeshima Y, Lee T, Awano H, Yagi M, Kurahashi H, Morioka I, Toda T, Matsuo M, Iijima K
Organizer
the 13th International Congress of Human Genetics
Place of Presentation
Kyoto, Japan
Year and Date
2016-04-03
Related Report
Int'l Joint Research
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[Presentation] Comparison between fetal and postnatal imaging in the detection of cerebral abnormalities of congenital cytomegalovirus infection.2015
Author(s)
Sugioka Y, Nishii T, Sugimoto K, Sugimura K, Akasaka Y, Tanimura K, Tairaku S, Yamada H, Morioka I, Iijima K
Organizer
the 15th Annual Meeting of Asian & Oceanic Society for Pediatric Radiology
Place of Presentation
Tokyo, Japan
Year and Date
2015-06-11
Related Report
Int'l Joint Research
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[Presentation] Efficacy of Antiviral Treatment on Abnormal Auditory Brainstem Responses in Congenital Cytomegalovirus-Infected Infants.2015
Author(s)
Morioka I, Nishida K, Yamashita D, Kobayashi Y, Nakamachi Y, Imanishi T, Deguchi M, Tanimura K, Kawano S, Nibu KI, Iijima K, Yamada H
Organizer
Pediatric Academic Society Annual Meeting
Place of Presentation
San Diego, USA
Year and Date
2015-04-25
Related Report
Int'l Joint Research
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[Presentation] Incidence of Sequelae in Symptomatic Congenital Cytomegalovirus-infected Infants After Introduction of Newborn Urine Screening and Antiviral Treatment.2015
Author(s)
Nishida K, Morioka I, Kobayashi Y, Nakamachi Y, Imanishi T, Deguchi M, Tanimura K, Funakoshi T, Ohashi M, Inoue N, Iijima K, Yamada H
Organizer
the 5th International Congenital Cytomegalovirus Conference and the 15th International CMV/Betaherpes Workshop
Place of Presentation
Brisbane, Australia
Year and Date
2015-04-20
Related Report
Int'l Joint Research
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[Presentation] Follow-Up Study of Infants With Symptomatic Congenital Cytomegalovirus Infection Diagnosed by Urine Screening Soon After Birth.2015
Author(s)
Nishida K, Morioka I, Nakamachi Y, Kobayashi Y, Okazaki Y, Noguchi Y, Deguchi M, Tanimura K, Kawano S, Iijima K, Yamada H.
Organizer
the 11th Congress of Asian Society for Pediatric Research
Place of Presentation
Osaka, Japan
Year and Date
2015-04-15
Related Report
Int'l Joint Research
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[Presentation] Low total IgM values and high cytomegalovirus loads in the blood of newborns with symptomatic congenital cytomegalovirus infection.2014
Author(s)
Morioka I, Kobayashi Y, Koda T, Nakamachi Y, Okazaki Y, Noguchi Y, Tairaku S, Tanimura K, Deguchi M, Ebina Y, Kawano S, Iijima K, Yamada H.
Organizer
39th Annual International Herpesvirus Workshop
Place of Presentation
Kobe, Japan
Year and Date
2014-07-19 – 2014-07-23
Related Report
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[Presentation] The IgG avidity value for the prediction of congenital cytomegalovirus infection in a prospective cohort study2014
Author(s)
Ebina Y, Minematsu T, Sonoyama A, Morioka I, Inoue N, Tairaku S, Nagamata S, Tanimura K, Morizane M, Deguchi M, Yamada H.
Organizer
39th Annual International Herpesvirus Workshop
Place of Presentation
Kobe, Japan
Year and Date
2014-07-19 – 2014-07-23
Related Report
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[Presentation] A single nucleotide polymorphism in the NKG2D gene is associated with clinical outcome of congenital cytomegalovirus infection at birth2014
Author(s)
Taniguchi R, Koyano S, Suzutani T, Goishi K, Ito Y, Morioka I, Nakamura H, Yamada H, Oka A, Inoue N.
Organizer
39th Annual International Herpesvirus Workshop
Place of Presentation
Kobe, Japan
Year and Date
2014-07-19 – 2014-07-23
Related Report
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[Presentation] Characterizations of blood markers for cytomegalovirus (CMV) detection in newborns with congenital CMV infection2014
Author(s)
Morioka I, Kobayashi Y, Nakamachi Y, Okazaki Y, Noguchi Y, Ogi M, Chikahira M, Inoue N, Yamada H, Kawano S, Iijima K.
Organizer
Pediatric Academic Society and Society for Asian Pediatric Research Joint Meeting
Place of Presentation
Vancouver, Canada
Year and Date
2014-05-03 – 2014-05-06
Related Report
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