|Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|Outline of Final Research Achievements
There was a distinct population called side population cells about 1 to 2 % of human SCC cells, which was almost identical results with chemically induced mouse SCC. To determine the capacity of tumorigenesis of SP cells, I sorted 640 SP cells and non-SP cells, and tried to compare their tumorigenecity by in vitro assay, but non worked well. Next, I investigated the expression of CD98 in human SCC. About two third of SCC cells expressed CD98. To determine the relationship between SP cells and CD98 expression, I performed dual staining of Hoechst dye and CD98. Most of SP cells expressed CD98. I could not detect any SP cells in melanoma cell line or dermatofibrosarcoma protuberans cells.