Modification of radio-sensitivity of cancer by miRNA delivery taking advantage of the exosome.
| Project/Area Number |
26461882
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | University of Toyama |
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Principal Investigator |
Ogawa Ryohei 富山大学, 大学院医学薬学研究部(医学), 准教授 (60334736)
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| Co-Investigator(Kenkyū-buntansha) |
鍵谷 豪 北里大学, 医療衛生学部, 准教授 (30524243)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Akihiko 富山大学付属病院, 講師 (20377253)
MORII Akihiro 黒部市民病院, 泌尿器科, 部長 (20377279)
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| Research Collaborator |
DOI Nobutaka ニッポンジーン株式会社, 研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | エクソソーム / miRNA / 放射線感受性 / shRNA / HEK293細胞 |
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| Outline of Final Research Achievements |
In this research project, we focus on a small vesicle secreted out of a cell called exosome (Ex) as a vehicle to deliver miRNA molecules to a target tissue in the living body. Overexpression of two genes that is involved in biogenesis of Ex resulted in the increase amount of secreted Ex from the cells. In addition, we already obtained two different miRNA like molecules that increase radio-sensitivity of cells when introduced into cells. However, since we have not been able to clone a key gene that is involved in enclosure of RNA molecules into Ex, we could not examine the function of the gene.
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Report
(4 results)
Research Products
(11 results)
