| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
The in vivo T1-weighted contrasting abilities and signal decay behaviors of several nitroxyl contrast agents in mouse brain were compared. The amphiphilic 3-methoxy-carbonyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (MCP) and 4-hydroxyl-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL) had good initial uniform distribution in the brain and showed typical 2-phase signal decay profiles. Nitroxyl contrast agents equipped with a suitable lipophilic substitution group could be BBB-permeable functional contrast agents.
Daily variations of redox behavior in mouse brain after irradiation of X-ray or carbon-ion beams were tried to estimate based on the in vivo reduction rate of amphiphilic nitroxyl contrast agents, MCP and TEMPOL. Irradiation of ionized radiation to head could cause alternation of redox status in the brain 2 days after X-ray irradiation or 1 day after carbon-beam irradiation. Superoxide leakage from damaged mitochondria can be concerned due to increased decay rate of TEMPOL.
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