Development of a novel therapy targeting Wnt5a-related cell adhesion factor ALCAM
| Project/Area Number |
26461947
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
Kadoya Takayuki 広島大学, 原爆放射線医科学研究所, 講師 (20609763)
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| Co-Investigator(Kenkyū-buntansha) |
岡田 守人 広島大学, 原爆放射線医科学研究所, 教授 (70446045)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Wnt5a / 乳癌 / 分子標的治療 / 治療 / 分子標的 |
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| Outline of Final Research Achievements |
Wnt5a was positively expressed in 69 cases (39%) among 178 cases of invasive breast cancer. Wnt5a expression was strongly correlated with being estrogen receptor (ER)-positive (P < 0.001), and Wnt5a was barely expressed in ER-negative breast cancers. Analysis of the relationship between Wnt5a expression and malignancy in exclusively 153 cases of ER-positive breast cancer led to detection of significant correlations with lymph node metastasis (P < 0.001), nuclear grade (P = 0.004), and lymphatic invasion (P = 0.002). Relapse-free survival was shorter in cases of Wnt5a-positive breast cancer compared to Wnt5a-negative breast cancer cases (P = 0.024). MCF7 cells forced to constitutively express Wnt5a showed significantly enhanced migratory capacity as compared to control cells. In order to clarify the mechanism, a DNA microarray analysis was carried out. Activated leukocyte cell adhesion molecule (ALCAM) was identified as an Wnt5a-dependent expression molecule.
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Report
(4 results)
Research Products
(4 results)
