| Project/Area Number |
26462046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
IKUTA Yoshiaki 熊本大学, 医学部附属病院, 非常勤診療医師 (70452894)
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| Co-Investigator(Kenkyū-buntansha) |
近本 亮 熊本大学, 医学部附属病院, 准教授 (10419640)
馬場 秀夫 熊本大学, 大学院生命科学研究部(医), 教授 (20240905)
林 洋光 熊本大学, 医学部附属病院, 非常勤診療医師 (80625773)
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| Research Collaborator |
KUROKI Hideyuki 熊本大学, 医学部附属病院, 非常勤診療医師 (50594876)
MIYAKE Keisuke 熊本大学, 大学院生命科学研究部(医), リサーチスペシャリスト
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 門脈血流低下 / 肝萎縮 / TSP-1 / 肝細胞増殖抑制 / 肝細胞アポトーシス誘導 / 肝類洞内皮細胞 / 増殖抑制 / アポトーシス / 肝血流低下 / 肝肥大 / 増殖 / Smad2 |
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| Outline of Final Research Achievements |
Liver atrophy was induced right portal vein ligation of wild type mouse. PCNA expression elevated at 6 hour after portal vein ligation and decreased thereafter in ligated liver (atrophic liver). TUNEL stained nuclei in liver cells increased over time. TSP-1 expression in cytoplasm of ligated liver increased since 6 hour after portal vein ligation. cDNA microarray assay was performed on ligated and non-ligated human liver for therapeutic hepatectomy. TSP-1 was most induced among 132 genes in ligated liver than in none ligated liver.
These results show hypoperfusion of portal vein induce TSP-1 expression in hepatocyte and suppress proliferation and induce apoptosis of hepatocyte.
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