Fetal lung fragments implantation into fibrotic lung of a pig model

• Project/Area Number: 26462127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Kochi National Hospital (2016); The University of Tokushima (2014-2015)
• Principal Investigator: SAKIYAMA Shoji 独立行政法人国立病院機構高知病院(臨床研究部), 副院長室, 副院長 (60291986)
• Co-Investigator(Kenkyū-buntansha): 滝沢 宏光 徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (90332816) ; 川上 行奎 徳島大学, 病院, 特任講師 (00596249) ; 鳥羽 博明 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (40403745) ; 梶浦 耕一郎 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (60596253) ; 坪井 光弘 徳島大学, 病院, 助教 (10711872) ; 吉田 光輝 徳島大学, 病院, 講師 (30403710)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 肺の再生 / 胎生期肺 / 肺組織移植 / 肺線維症 / 豚 / ラット / ステロイド

Outline of Final Research Achievements

One of the approaches to lung regeneration and repair, we have noticed to use fetal lung tissues having great potential to further grow and differentiate, and made an experimental model. In the research of fetal lung tissues implantation into normal adult lungs, we showed that implanted fetal tissues were able to live and grow in adult lung using mouse, rat and pig model. The speed of differentiation and growth of implanted fetal tissues dependents on the kind. Speed of the growth and differentiation is slower in the pigs than the rodents. Administration of glucocorticoid to recipients after fetal lung tissues implantation facilitated the development and growth of implanted fetal lung tissues. The quantity of elastin in implanted fetal tissues was increased by glucocorticoid. These effects depended on the duration of the glucocorticoid use. Although fetal lung tissue could survive and differentiate in the fibrotic lung in the rats, in the pig model the matter have not been proved yet.

Research Products

• [Journal Article] Lung regeneration by fetal lung tissue implantation in a mouse pulmonary emphysema model (2016)
  • Author(s): Uyama K, Sakiyama S, Yoshida M, MD, Kenzaki K, Toba H, Kawakami Y, Okumura K, Takizawa H, Kondo K and Tangoku A.
  • Journal Title: J Med Invest Volume: 63
  • NAID: 130005245852
  • Peer Reviewed / Open Access
• [Presentation] Lung regeneration: Glucocorticoid administration facilitates differentiation of rat fetal lung fragments into adult rat lung parenchyma. (2014)
  • Author(s): S. Sakiyama, M. Yoshida, Y. Kawakami, K Kenzaki, M. Tsuboi, K. Kajiura, Y. Nakagawa, H. Takizawa, K. Kondo, A. Tangoku
  • Organizer: The 2014 American Thoracic Society International Conference
  • Place of Presentation: San Diego Convension Center（San Diego,USA）
  • Year and Date: 2014-05-16 – 2014-05-21
• [Presentation] 胎生期肺組織移植における気道系の分化とbranchingに関する検討 (2014)
  • Author(s): 先山正二、鳥羽博明、監崎孝一郎、坪井光弘、梶浦耕一郎、中川靖士、吉田光輝、川上行奎、 滝沢宏光、近藤和也、丹黒 章
  • Organizer: 第37回日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2014-04-14 – 2014-04-15