New biomarker development by the microRNA analysis with blood sample of the lung cancer patients

• Project/Area Number: 26462138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Tokyo Medical University
• Principal Investigator: Ohira Tatsuo 東京医科大学, 医学部, 教授 (40317847)
• Co-Investigator(Kenkyū-buntansha): 池田 徳彦 東京医科大学, 医学部, 主任教授 (70246205)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 肺癌 / バイオマーカー / マイクロRNA

Outline of Final Research Achievements

The microRNA is the small RNA molecules which does not encode protein. The object of this study is to examine whether microRNA is effective as biomarker of the lung cancer. I analyzed approximately 900 microRNA comprehensively. Then, I analyzed specific microRNA. As a result, I detected a decrease of five kinds of microRNA and five kinds of increase. Based on this result, I started examination about possibility as the biomarker whether the detection with the blood was possible. As a result of having searched the clinicopathologic factor which prescribed a variation rate of detection in blood, the result that the volume of tumor was related to was provided. By the gene analysis derived from a tumor in the blood, it was shown by the early lung cancer that became a target of the operation that the detection was difficult. I continue study about biomarker for lung cancer with microRNA.

Research Products

• [Journal Article] Skin fluorescence following photodynamic therapy with NPe6 photosensitizer (2017)
  • Author(s): Ohtani K, Usuda J, Ogawa E, Maehara S, Imai K, Kudo Y, Ono S, Shigefuku S, Eriguchi D, Inoue T, Maeda J, Yoshida K, Hagiwara M, Kakihana M, Kajiwara N, Ohira T, Arai T, Ikeda N
  • Journal Title: Photodiagnosis Photodyn Ther Volume: 20 Pages: 210-214
  • DOI: 10.1016/j.pdpdt.2017.10.001
  • Peer Reviewed
• [Journal Article] Repression of Smad3 by Stat3 and c-Ski/SnoN induces gefitinib resistance in lung adenocarcinoma (2017)
  • Author(s): Makino Y, Yoon JH, Bae E, Kato M, Miyazawa K, Ohira T, Ikeda N, Kuroda M, Mamura M.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 484 Pages: 269-277
  • DOI: 10.1016/j.bbrc.2017.01.093
  • NAID: 120006027488
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tumor volume determines the feasibility of cell-free DNA sequencing for mutation detection in non-small cell lung cancer. (2016)
  • Author(s): Ohira T, Sakai K, Matsubayashi J, Kajiwara N, Kakihana M, Hagiwara M, Hibi M, Yoshida K, Maeda J, Ohtani K, Nagao T, Nishio K, Ikeda N.
  • Journal Title: Cancer Sci. Volume: 107 Pages: 1660-1666
  • DOI: 10.1111/cas.13068
  • Peer Reviewed
• [Journal Article] Detection of the T790M mutation of EGFR in plasma of advanced non-small cell lung cancer patients with acquired resistance to tyrosine kinase inhibitors (West Japan oncology group 8014LTR study). (2016)
  • Author(s): Takahama T, Sakai K, Takeda M, Azuma K, Hida T, Hirabayashi M, Oguri T, Tanaka H, Ebi N, Sawa T, Bessho A, Tachihara M, Akamatsu H, Bandoh S, Himeji D, Ohira T, Shimokawa M, Nakanishi Y, Nakagawa K, Nishio K.
  • Journal Title: Oncotarget Volume: 7 Pages: 58492-58499
  • DOI: 10.18632/oncotarget.11303
  • Peer Reviewed / Open Access
• [Journal Article] Association between high-resolution computed tomography findings and the IASLC/ATS/ERS classification of small lung adenocarcinomas in Japanese patients (2015)
  • Author(s): Kudo Y, Matsubayashi J, Saji H, Akata S, Shimada Y, Kato Y, Kakihana M, Kajiwara N, Ohira T, Nagao T, Ikeda N.
  • Journal Title: Lung Cancer Volume: 90 Pages: 47-54
  • DOI: 10.1016/j.lungcan.2015.07.007
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Survival outcomes for oligometastasis in resected non-small cell lung cancer. (2015)
  • Author(s): Shimada Y, Saji H, Kakihana M, Kajiwara N, Ohira T, Ikeda N.
  • Journal Title: Asian Cardiovasc Thorac Ann. Volume: 23 Pages: 937-944
  • DOI: 10.1177/0218492315596463
  • Peer Reviewed
• [Journal Article] A proteomic profiling of laser-microdissected lung adenocarcinoma cells of early lepidic-types. (2015)
  • Author(s): Kato Y, Nakamura H, Tojo H, Nomura M, Nagao T, Kawamura T, Kodama T, Ohira T, Ikeda N, Fehniger T, Marko-Varga G, Nishimura T, Kato H.
  • Journal Title: Clin Transl Med. Volume: 4 Pages: 64-64
  • DOI: 10.1186/s40169-015-0064-3
  • Peer Reviewed
• [Journal Article] Prognostic Factors for Survival After Recurrence in Patients With Completely Resected Lung Adenocarcinoma: Important Roles of Epidermal Growth Factor Receptor Mutation Status and the Current Staging System. (2015)
  • Author(s): Kudo Y, Shimada Y, Saji H, Kato Y, Yoshida K, Matsubayashi J, Nagase S, Kakihana M, Kajiwara N, Ohira T, Nagao T, Ikeda N.
  • Journal Title: 10.1016/j.cllc.2015.04.005. Volume: 16 Pages: 213-221
  • DOI: 10.1016/j.cllc.2015.04.005
  • Peer Reviewed
• [Journal Article] Factors Influencing the Diagnostic Accuracy of Identifying the Histologic Type of Non--Small-Lung Cancer With Small Samples. (2015)
  • Author(s): Oikawa T, Ohira T, Matsubayashi J, Konaka C, Ikeda N.
  • Journal Title: Clin Lung Cancer. Volume: 16 Pages: 374-378
  • DOI: 10.1016/j.cllc.2015.03.007
  • Peer Reviewed
• [Journal Article] Combination effect of photodynamic therapy using NPe6 with pemetrexed for human malignant pleural mesothelioma cells. (2015)
  • Author(s): Maehara S, Usuda J, Ishizumi T, Ichinose S, Ohtani K, Inoue T, Imai K, Furumoto H, Kudo Y, Kajiwara N, Ohira T, Ikeda N.
  • Journal Title: Int J Oncol. Volume: 46 Pages: 741-749
  • DOI: 10.3892/ijo.2014.2746
  • Peer Reviewed / Open Access
• [Journal Article] Clinical usefulness of gefitinib for non-small-cell lung cancer with a double epidermal growth factor receptor mutation. (2015)
  • Author(s): Oikawa T, Ohira T, Otani K, Hagiwara M, Konaka C, Ikeda N.
  • Journal Title: Mol Clin Oncol. Volume: 3 Pages: 329-333
  • Peer Reviewed / Open Access
• [Journal Article] High-speed 3-dimensional imaging in robot-assisted thoracic surgical procedures (2014)
  • Author(s): Kajiwara N, Akata S, Hagiwara M, Yoshida K, Kato Y, Kakihana M, Ohira T, Kawate N, Ikeda N
  • Journal Title: Ann Thorac Surg. Volume: 97 Pages: 2182-2184
  • DOI: 10.1016/j.athoracsur.2013.08.026
  • Peer Reviewed / Open Access
• [Journal Article] High-quality 3-dimensional image simulation for pulmonary lobectomy and segmentectomy: results of preoperative assessment of pulmonary vessels and short-term surgical outcomes in consecutive patients undergoing video-assisted thoracic surgery (2014)
  • Author(s): Hagiwara M, Shimada Y, Kato Y, Nawa K, Makino Y, Furumoto H, Akata S, Kakihana M, Kajiwara N, Ohira T, Saji H, Ikeda N.
  • Journal Title: Eur J Cardiothorac Surg. Volume: 46 Pages: 120-126
  • DOI: 10.1093/ejcts/ezu375
  • Peer Reviewed / Open Access
• [Presentation] Liquid based cytology (LBC) specimens were useful for EGFR mutation test (2017)
  • Author(s): Ohira T, Matsubayashi J, Maehara S, Maeda J, Yoshida K, Hagiwara M, Kakihana M, Okano T, Kajiwara N, Nagao T, Ikeda N
  • Organizer: IASLC 18th World Conference on Lung Cancer
  • Int'l Joint Research
• [Presentation] 肺癌に対する網羅的遺伝子解析 (2017)
  • Author(s): 大平達夫，濱中和嘉子，松浦久美，今井健太郎，前原幸夫，前田純一，吉田浩一，萩原優，垣花昌俊，梶原直央，池田徳彦
  • Organizer: 第117回日本外科学会定期学術集会
• [Presentation] The specimen sampling and handling for tailor maid treatment of the lung cancer (2016)
  • Author(s): Ohira T, Matsubayashi J, Otani K, Maeda J, Yoshida K, Hagiwara M, Kakihana M, Kajiwara N, Nagao T, Ikeda N
  • Organizer: The 19th International Congress of Cytology
  • Place of Presentation: Yokohama
  • Int'l Joint Research
• [Presentation] The Feasibility of Cell-Free DNA Sequencing for Mutation Detection in Non-Small Cell Lung Cancer Was Detemined by Tumor Volume (2016)
  • Author(s): Ohira T, Sakai K, Maehara S, Maeda J, Yoshida K, Hagiwara M, Kakihana M, Okano T, Kajiwara N, Nishio K, Ikeda N
  • Organizer: 17th World Conference on Lung Cancer
  • Place of Presentation: Vienna, Austria
  • Int'l Joint Research
• [Presentation] 分子生物学研究が呼吸器外科の治療にもたらすもの (2016)
  • Author(s): 大平 達夫，今井健太郎，前原 幸夫，大谷 圭志，前田 純一，吉田 浩一，萩原 優，垣花 昌俊，梶原 直央，池田 徳彦
  • Organizer: 第33回日本呼吸器外科学会総会
  • Place of Presentation: 京都
• [Presentation] 呼吸器内視鏡検査で得られた細胞診検体を用いた遺伝子解析の有効性 (2016)
  • Author(s): 大平 達夫，今井健太郎，前原 幸夫，大谷 圭志，前田 純一，吉田 浩一，萩原 優，垣花 昌俊，岡野 哲也，梶原 直央，池田 徳彦
  • Organizer: 第39回日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] 肺癌手術患者に対するリキッドバイオプシーの有用性と限界 (2016)
  • Author(s): 大平 達夫，坂井 和子，前原 幸夫，前田 純一，吉田 浩一，萩原 優，垣花 昌俊，岡野 哲也，松林 純，梶原 直央，長尾 俊孝，西尾 和人，池田 徳彦
  • Organizer: 第57回日本肺癌学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 化学放射線治療後に再発を呈した腺様嚢胞癌に対するサルベージ手術 (2015)
  • Author(s): 大平達夫、加藤靖文、嶋田善久、前原幸夫、大谷圭志、片場寛明、前田純一、吉田浩一、萩原 優、梶原直央、池田徳彦
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2015-11-26
• [Presentation] 気管腺様嚢胞癌に対するサルベージ手術 (2015)
  • Author(s): 大平達夫、加藤靖文、嶋田善久、前原幸夫、前田純一、吉田浩一、萩原 優、垣花昌俊、梶原直央、池田徳彦
  • Organizer: 第68回日本胸部外科学会定期学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2015-10-20
• [Presentation] Clinicopathologic and biological characteristics of young age non-small cell lung cancer (2015)
  • Author(s): Ohira T, Ohtani K, Kataba H, Maehara S, Shimada Y, Maeda J, Yohida K, Kato Y, Hagiwara M, Kakihana M, Kajiwara N, Ikeda N
  • Organizer: 16th World Conference on Lung Cancer
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2015-09-07
  • Int'l Joint Research
• [Presentation] 東京医科大学における肺癌診療・研究の現状 (2015)
  • Author(s): 大平達夫
  • Organizer: 第4回医薬工3大学包括連携推進シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-06-20
  • Invited
• [Presentation] 肺癌薬物療法のための呼吸器内視鏡検査の重要性 (2015)
  • Author(s): 大平達夫、片場寛明、大谷圭志、前田純一、吉田浩一、加藤靖文、萩原 優、垣花昌俊、長瀬清亮、梶原直央、池田徳彦
  • Organizer: 第38回日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-06-11
• [Presentation] 気管腺様嚢胞癌に対する化学放射線治療後再発に対する再手術 (2015)
  • Author(s): 大平達夫、加藤靖文、嶋田善久、大澤潤一郎、前田純一、吉田浩一、萩原 優、垣花昌俊、梶原直央、池田徳彦
  • Organizer: 第32回日本呼吸器外科学会総会
  • Place of Presentation: 高松
  • Year and Date: 2015-05-15
• [Presentation] ロボット支援手術は胸腔鏡下手術より有用か？ (2015)
  • Author(s): 大平達夫、大谷圭志、片場寛明、前田純一、吉田浩一、加藤靖文、萩原 優、垣花昌俊、梶原直央、池田徳彦
  • Organizer: 第115回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-16
• [Presentation] 癌治療の進歩によって緩和ケアへのギアチェンジは変わったか (2014)
  • Author(s): 大平 達夫、前原 幸夫，嶋田 善久，大谷圭志，前田 純一，吉田 浩一，加藤 靖文，萩原 優，垣花 昌俊，長瀬 清亮，梶原 直央，池田 徳彦
  • Organizer: 第55回日本肺癌学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-11-14 – 2014-11-16
• [Presentation] 当科における呼吸器外科医育成プログラム (2014)
  • Author(s): 大平 達夫、前原 幸夫，嶋田 善久，及川 武史，大谷 圭志，前田 純一，吉田 浩一，加藤 靖文，垣花 昌俊，梶原 直央，池田 徳彦
  • Organizer: 第31回日本呼吸器外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-29 – 2014-05-30
• [Presentation] 肺癌治療成績向上のためのバイオマーカー研究 (2014)
  • Author(s): 大平 達夫、及川 武史，山口 学，加藤 靖文，前田 純一，吉田 浩一，萩原 優，垣花 昌俊，梶原 直央，筒井 英光，池田 徳彦
  • Organizer: 第10回日本臨床プロテオーム研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-10
• [Presentation] Histological diagnosis of lung cancer using small biopsy samples (2014)
  • Author(s): Ohira T, Oikawa T, Otani K, Yoshida K, Kato Y, Maeda J, Hagiwara M, Nagase S, Kakihana M, Kajiwara N, Ikeda N
  • Organizer: 18th WCBIP/ WCBE World Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2014-04-14 – 2014-04-15
• [Presentation] 気管支鏡下レーザー治療における安全性の確保と合併症対策 (2014)
  • Author(s): 大平 達夫、大谷 圭志，前原 幸夫，吉田 浩一，加藤 靖文，前田 純一，萩原 優，垣花 昌俊，長瀬 清亮，梶原 直央，池田 徳彦
  • Organizer: 第37回日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-14 – 2014-04-15
• [Remarks] 東京医科大学呼吸器・甲状腺外科学分野（旧外科学第一講座）
  • URL: http://team.tokyo-med.ac.jp/kokyukigeka/index.html