Investigation of stability of calcified plaque by miRNA profiling
| Project/Area Number |
26462169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
KATANO Hiroyuki 名古屋市立大学, 大学院医学研究科, 准教授 (30295612)
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| Co-Investigator(Kenkyū-buntansha) |
山田 和雄 名古屋市立大学, 大学院医学研究科, 教授 (90150341)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | carotid plaque / calcification / microRNA / microarray / PCR / carotid endarterectomy / calcium score / miRNA / RT-PCR |
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| Outline of Final Research Achievements |
Ten plaques removed from carotid endarterectomy patients were classified into high- and low-calcified plaques on the basis of Agatston calcium score. Microarray analysis for miRNA profiles was performed followed by validation with miRNA quantitative real-time (qRT) PCR analysis. Genes which satisfied total gene signal (TGS) >50 and [Log2 ratio <-1 or >1 were selected. Among carefully selected 70 angiogenesis or calcification-related transcripts, reliably expressed miRNAs marking median and high context scores with miRSearch and FDR <0.1 by the two-group comparison were also extracted .In high-calcified carotid plaques, specific profile for miRNA may be shown and expression of hsa-miR-4530 and hsa-miR-133b had inverse correlation with calcium score in plaques, suggesting miRNA may play a modulating role in calcification in plaque and plaque stability.
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Report
(4 results)
Research Products
(2 results)
