Investigatation of chronic inflammation in bone by gastro-intestinal-bone axis

• Project/Area Number: 26462316
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Wakayama Medical University
• Principal Investigator: Mikitaka Iguchi 和歌山県立医科大学, 医学部, 講師 (40336889)
• Co-Investigator(Kenkyū-buntansha): 山本 悠太 和歌山県立医科大学, 医学部, 講師 (00580672) ; 一瀬 雅夫 和歌山県立医科大学, 医学部, 教授 (50143425) ; 上山 敬司 和歌山県立医科大学, 医学部, 教授 (50264875) ; 上田 和樹 和歌山県立医科大学, 医学部, 博士研究員 (50405437) ; 鶴尾 吉宏 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (90207449)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 胃切除 / 骨代謝異常 / 炎症 / 胃切除動物 / マイクロアレイ / 直腸 / リンパ球浸潤 / LC-MS / リンパ球 / 浸潤 / 骨代謝 / 組織化学 / 遺伝子解析 / 臓器連関

Outline of Final Research Achievements

We reported the abnormality of bone metabolism in gastrectomized rats. However, the effects of gastrectomy in multiple organs had not been clear. We examined the effect of gastrectomy on rat livers with analysis of gene expression, biochemistry, and histological changes.
Microarray analysis identified 573 differential expressed genes in gastrectomized rat liver, and pathway analysis indicated statistically relationship between these genes and steroid signaling and lipid metabolism. In gastrectomized rats, serum ALT was increased, and small droplets were observed in hepatocyte. Furthermore, lethal events were significantly happened in gastrectomy rats.
The case reports about liver dysfunction after gastrectomy have been reported, this study may explain the mechanisms of these cases.

Research Products

• [Journal Article] Serum pepsinogen levels can quantify the risk of development of metachronous gastric cancer after endoscopic resection. (2016)
  • Author(s): Iguchi M, Kato J, Fukatsu K, Yamamoto Y, Nakachi K, Yoshida T, Mori Y, Maeda Y, Moribata K, Shingaki N, Niwa T, Deguchi H, Inoue I, Maekita T, Tamai H, Ichinose M.
  • Journal Title: Int J Cancer. Volume: . Issue: 5 Pages: 1150-1156
  • DOI: 10.1002/ijc.30145
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Evidence-based clinical practice guidelines for peptic ulcer disease 2015 (2016)
  • Author(s): Satoh K, Yoshino J, Akamatsu T, Itoh T, Kato M, Kamada T, Takagi A, Chiba T, Nomura S, Mizokami Y, Murakami K, Sakamoto C, Hiraishi H, Ichinose M, Uemura N, Goto H, Joh T, Miwa H, Sugano K, Shimosegawa T
  • Journal Title: Journal of Gastroenterol Volume: 51 Issue: 3 Pages: 177-194
  • DOI: 10.1007/s00535-016-1166-4
  • Peer Reviewed
• [Journal Article] Efficacy of spraying l-menthol solution during endoscopic treatment of early gastric cancer: a phase III, multicenter randomized, double-blind, placebo-controlled study (2014)
  • Author(s): Fujishiro M, Kaminishi M, Hiki N, IOda I, Fujisaki J, Uedo N, Kaise M, Tanabe S, Iguchi M, Matsuhashi N, Nomura S, Tajiri H, Yahagi N, Suzuki H
  • Journal Title: J Gastroenterol Volume: 49 Pages: 446-454
  • Peer Reviewed
• [Journal Article] Cancer development based on chronic active gastritis and resulting gastric atrophy as assessed by serum levels of pepsinogen and Helicobacter pylori antibody titer, (2014)
  • Author(s): Yoshida T, Kato, Inoue I, Yoshimura N, Deguchi H, Mukoubayashi C, Oka M, Watanabe M, Enomoto S, Niwa T, Maekita T, Iguchi M, Tamai H, Ushijima T, Ichinose M
  • Journal Title: Int. J. Cancer Volume: 134 Pages: 1445-1457
  • Peer Reviewed
• [Journal Article] Nrf2-induceding anti-oxidation stress response in the liver - New beneficial effect of Lansoprazole (2014)
  • Author(s): Yamashita Y, Ueyama T, Nishi T, Yamamoto Y, Kawakoshi A, Sunami S, Iguchi M, Tamai H, Ueda K, Ito T, Tsuruo Y, Ichinose M
  • Journal Title: PLoS ONE Volume: 9(5) Issue: 5 Pages: e97419-e97419
  • DOI: 10.1371/journal.pone.0097419
  • Peer Reviewed / Open Access
• [Journal Article] 胃癌取り扱い規約・胃癌治療ガイドライン，取り扱い規約・ガイドラインを作成する意義：概論 (2014)
  • Author(s): 井口幹崇，一瀬雅夫
  • Journal Title: 日本臨床，最新胃癌学 Volume: vol.72, Suppl Pages: 231-234
• [Presentation] 「胃癌治療後のHP除菌（ESD後と胃切除後）」，ESD後異時性胃癌発生の危険因子，Hp除菌による抑制効果の検討 (2014)
  • Author(s): 井口幹崇，森畠康策，加藤 順
  • Organizer: JDDW
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-15