Prevention of induction of quinolone-resistance by inhibition of drug efflux pump in uropathogenic E. coli
| Project/Area Number |
26462455
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Hyogo Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
東郷 容和 兵庫医科大学, 医学部, 講師 (20611370)
山崎 栄樹 帯広畜産大学, 畜産学部, 助教 (40514708)
倉園 久生 帯広畜産大学, 畜産学部, 教授 (90186487)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 尿路病原性大腸菌 / キノロン耐性 / セフェム耐性 / 薬剤排出ポンプ / シークエンス / QRDR遺伝子変異 / ホールゲノムシークエンス / 大腸菌 / 尿路病原性 |
|---|
| Outline of Final Research Achievements |
We induced developed fluoroquinolone-resistant strains by the exposure to fluoroquinolone, followed by the final development of high-resistant isolates. In the developed fluoroquinolone-resistant isolates, additional mutations were found in gyrA, parC and parE. Further, quantitative analysis of mRNA expression of efflux pumps and their regulators expression revealed remarkable increase of marA expression, which is a positive regulator of efflux pump.
Another interesting finding was that these developed fluoroquinolone-resistant E. coli strains showed resistance against several cephalosporin as 8 to 32 times higher MIC than the original strains. We have started the whole-genome sequence of these strains to find the mutation which are different from their original strains.
|
Report
(4 results)
Research Products
(2 results)
