Development of allo-activated macrophage suppression method aiming at efficiency of Intravenous Immunoglobulin therapy

• Project/Area Number: 26462466
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Osaka Medical College
• Principal Investigator: Nomi Hayahito 大阪医科大学, 医学部, 准教授 (80418938)
• Co-Investigator(Kenkyū-buntansha): 東 治人 大阪医科大学, 医学部, 教授 (40231914)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アロ活性化マクロファージ / 免疫グロブリン大量療法 / 同種異型移植 / 免疫抑制剤 / ｱﾛ活性化ﾏｸﾛﾌｧｰｼﾞ / 腎移植 / マウス / 急性拒絶反応 / IVIG / 抗体関連型拒絶 / 免疫抑制

Outline of Final Research Achievements

Meth A fibro-sarcoma cells transplanted into allogeneic C57Bl/6 mice peritoneal cavities proliferate initially, and then Meth A are rejected out from the mice. The secondary transplantations of Meth A were caused antibody related acute rejections in C57Bl/6 mice. We collected the peritoneal exudate cells (PEC) from these secondary transplantations (S-PEC). And we investigated cytotoxic activity of S-PEC in complement immobilized conditions with 51Cr releasing assays.
We isolated each fractions of S-PEC with FACS cell-sorter, and confirmed the highest allogeneic cytotoxicity was seen in allograft induced macrophages (AIM) fractions. And, we found the activities of AIM were attenuated by massive nonspecific IgG antibodies, in vitro. The removal of AIM in S-PEC also attenuates the cytotoxic activity of S-PEC. It is presumed IVIG acts on cytotoxic activity of AIM. There is a necessity to evaluate the inhibitory effect of IVIG on AIM without compromising complement.

Research Products

• [Presentation] Complications Around The Operation of Living Donor Renal Transplantation in Our Hospital (2016)
  • Author(s): HAYAHITO NOMI, HAJIME HIRANO, TOMOHISA MATSUNAGA, RYOUICHI MAENOSONO, HIROFUMI UEHAR, KAZUMASA KOMURA, KIYOSHI TAKAHARA, TERUO INAMOTO, SATOSHI KIYAMA, HARUHITO AZUMA
  • Organizer: Transplantation Science Symposium(TSS) Asian Regional Meeting 2016
  • Place of Presentation: KFC Hall(東京都墨田区)
• [Presentation] アロ移植細胞拒絶におけるマクロファージのエフェクター細胞としての役割 (2016)
  • Author(s): 能見勇人、吉川勇希、前之園 良一、松永和久、市橋 淳、小林大介、辻野拓也、反田直希、内本泰三、上原博史、平野一、高原 健、稲元輝生、東 治人
  • Organizer: 第66回日本泌尿器科学会中部総会
  • Place of Presentation: 四日市市文化会館(三重県四日市)
• [Presentation] アロ移植細胞拒絶におけるアロ活性化マクロファージの攻撃細胞としての役割 (2015)
  • Author(s): 能見勇人, 平野 一,松永 知久,前之園 良一,吉川勇希, 辻野拓也,斉藤賢吉,高井朋聡,内本泰三,高原 健,稲元輝生,木山 賢,東 治人
  • Organizer: 日本泌尿器科学科
  • Place of Presentation: 金沢都ホテル(石川県金沢市)
  • Year and Date: 2015-04-18 – 2015-04-19
• [Presentation] アロ移植細胞拒絶における アロ活性化マクロファージの攻撃細胞としての役割 (2015)
  • Author(s): 能見 勇人, 平野 一,松永 知久,前之園良一,吉川勇希, 辻野拓也, 斉藤賢吉, 高井朋聡, 内本泰三,高原 健, 稲元輝生, 木山 賢, 東 治人
  • Organizer: 第103回日本泌尿器科学会総会
  • Place of Presentation: 金沢都ホテル（石川県金沢市）
  • Year and Date: 2015-04-18
• [Presentation] アロ活性化マクロファージによるアロ細胞拒絶機構に関する検討 (2014)
  • Author(s): 能見勇人, 平野 一,前之園 良一,西本優大,松永知久,吉川勇希,斉藤賢吉,内本泰三,南 幸一郎,木山 賢,東 治人
  • Organizer: 日本移植学会
  • Place of Presentation: 京王プラザホテル(東京都新宿区)
  • Year and Date: 2014-09-11