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The functional analysis of spindle body using the human ovum of each generation to be removed during surgery and nuclear transplantation.

Research Project

Project/Area Number 26462473
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionIwate Medical University (2016)
Akita University (2014-2015)

Principal Investigator

KUMAGAI JIN  岩手医科大学, 医学部, 教授 (60333936)

Co-Investigator(Kenkyū-buntansha) 寺田 幸弘  秋田大学, 医学(系)研究科(研究院), 教授 (10260431)
宇都宮 裕貴  東北大学, 大学病院, 准教授 (10359507)
佐藤 直樹  秋田大学, 医学(系)研究科(研究院), 准教授 (40447199)
白澤 弘光  秋田大学, 医学部, 医員 (60598019)
佐藤 亘  秋田大学, 医学部, 助教 (10726441)
椛嶋 克哉  秋田大学, 医学部, 技術系補佐員 (30615422)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords卵子 / 蛍光免疫染色 / 卵子の輸送 / 電界撹拌 / ヒト卵子 / 体外培養 / 体癌患者 / 電界撹拌法 / 卵子輸送 / 加齢 / 卵巣 / 紡錘体 / 核移植
Outline of Final Research Achievements

Using non-contact alternating-current electric-field mixing of microdroplets, we developed the economical fluorescence immunostaining of the human ovum with time efficiency. We could stain the mouse ovum by this method using the anti-tubulin antibody which was the first antibody by 16,000 times dilution that 80 times as large as recommended dilution and could shorten discrimination time until five minutes. It is expected the saving precious antibodies and shortening the staining time in a future study.
On the other hand, concerning the human ovum transportation, in vitro maturation rate of human ovum significantly decreased in the immature ovum transportation case of 250km between Akita and Sendai, but improved it by stabilizing a vapor phase using a cell porter in a transportation container. This result is expected to bring the application to the ovum freeze of women with malignant tumor.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (4 results)

  • [Journal Article] Corrigendum: Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating- current electric- field mixing of microdroplets.2016

    • Author(s)
      Shirasawa H, Kumagai J, Sato E, Kabashima K, Kumazawa Y, Sato W, Miura H, Nakamura R, Nanjo H, Minamiya Y, Akagami Y, Terada Y.
    • Journal Title

      Scientic Reports

      Volume: 7 Issue: 1 Pages: 6-6

    • DOI

      10.1038/srep41552

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets.2015

    • Author(s)
      Shirasawa H,Kumagai J, Sato E, Kabashima K,Kumazawa Y,Sato W,Miura H,Nakamura R,Nanjo H, Minamiya Y,Akagami Y,Terada Y
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 15371-15371

    • DOI

      10.1038/srep15371

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ヒト卵胞液の大学病院間輸送方法および輸送後の体外成熟培養結果に関する検討.2015

    • Author(s)
      白澤弘光、 佐藤恵美子、 熊谷仁、 富樫嘉津恵、 下田勇輝、 佐藤亘、 熊澤由紀代、 井原基公、宇都宮裕貴 、八重樫伸生、 寺田幸弘
    • Organizer
      第56回日本卵子学会
    • Place of Presentation
      栃木
    • Year and Date
      2015-05-30
    • Related Report
      2015 Research-status Report
  • [Presentation] Novel rapid immunofluorescence staining method for the murine egg using the non-contact alternating current electric field mixing of the microdroplet.2015

    • Author(s)
      Shirasawa H, Kumagai J, Emiko Sato,Yukiyo Kumazawa,Wataru Sato,Kazue Togashi,Yukihiro Terada
    • Organizer
      IFFS/JSRM international meeting 2015
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-04-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 卵胞液の施設間輸送方法の改善が 摘出卵巣由来のヒト未成熟卵子に対する体外成熟培養結果へ与える影響に関する検討.2015

    • Author(s)
      白澤弘光、 熊谷仁、 富樫嘉津恵、 佐藤亘、 熊澤由紀代、 井原基公、 宇都宮裕貴、八重樫伸生、 寺田幸弘
    • Organizer
      第67回日本産か婦人科学会
    • Place of Presentation
      神奈川
    • Year and Date
      2015-04-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 約250km離れた施設間の卵胞液輸送後も,子宮体癌患者由来の未成熟卵子はMII期まで培養可能である.2014

    • Author(s)
      白澤弘光,熊谷仁,富樫嘉津恵,佐藤亘,熊澤由紀代,井原基公,宇都宮裕貴,八重樫伸生,寺田幸弘
    • Organizer
      第52 回東北生殖医学会
    • Place of Presentation
      秋田アトリオン
    • Year and Date
      2014-11-15
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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