brain damage after heat stroke in mice

• Project/Area Number: 26462769
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Showa University (2016-2017); Jikei University School of Medicine (2014-2015)
• Principal Investigator: Dohi Kenji 昭和大学, 医学部, 教授 (20301509)
• Co-Investigator(Kenkyū-buntansha): 大滝 博和 昭和大学, 医学部, 准教授 (20349062) ; 小川 武希 東京慈恵会医科大学, 医学部, 教授 (90119769) ; 宮本 和幸 昭和大学, 医学部, 助教 (80555087)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 熱中症 / 水素水 / モデル / マウス / 動物モデル / 酸化ストレス / 神経損傷 / フリーラジカル / 治療法

Outline of Final Research Achievements

The purpose of this project was to clarify the pathophysiology of neuroinflammation in mice heat stroke model. We developed mice heat stroke model which was not established. We also clarified the beneficial effect of the mineral supplement in heat stroke. The evaluation of the oxidative stress in heat stroke and the development of the new treatment using the hydrogen water is undergoing. We also found that heat stress cause intestinal tract damage. Basis of these results, we will clarify the relations with neuroinflammation and systemic inflammation in heat stroke.

Research Products

• [Journal Article] Oral rehydration solution increases SGLT1 and improves dehydration in a mouse heatstroke model. (2018)
  • Author(s): Miyamoto K, Ohtaki H, Takayasu H, Maeda A, Sasaki J, Honda K, Dohi K, Hayashi M.
  • Journal Title: Critical Care Medicine Volume: 46 Issue: 1 Pages: 252-252
  • DOI: 10.1097/01.ccm.0000528551.63676.f8
  • Peer Reviewed
• [Journal Article] Development of Neuroprotection and Neuroresuscitation in Neuroemergency Diseases (2018)
  • Author(s): Dohi Kenji
  • Journal Title: Showa Univ J Med Sci Volume: 29
  • Peer Reviewed / Open Access
• [Journal Article] Molecular hydrogen in the treatment of acute and chronic neurological conditions: mechanisms of protection and routes of administration (2017)
  • Author(s): kenji Dohi, Kazue Satoh, Kazuyuki Miyamoto, Shunsuke Mommma, Kenichiro Fukuda, Ryo Higuchi, Hirokazu Ohtaki, Williams A Banks
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 61 Issue: 1 Pages: 1-5
  • DOI: 10.3164/jcbn.16-87
  • NAID: 130006847653
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Intrapancreatic injection of human bone marrow-derived mesenchymal stem/stromal cells alleviates hyperglycemia and modulates the macrophage state in streptozotocin-induced type 1 diabetic mice. (2017)
  • Author(s): Murai N, Ohtaki H, Watanabe J, Xu Z, Sasaki S, Yagura K, Shioda S, Nagasaka S, Honda K, Izumizaki M.
  • Journal Title: PLoS One Volume: 12 Issue: 10 Pages: e0186637-e0186637
  • DOI: 10.1371/journal.pone.0186637
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pituitary adenylate cyclase-activating polypeptide promotes eccrine gland sweat secretion. (2017)
  • Author(s): Sasaki S, Watanabe J, Ohtaki H, Matsumoto M, Murai N, Nakamachi T, Hannibal J, Fahrenkrug J, Hashimoto H, Watanabe H, Sueki H, Honda K, Miyazaki A, Shioda S.
  • Journal Title: Br J Dermatol. Volume: 176 Issue: 2 Pages: 413-422
  • DOI: 10.1111/bjd.14885
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Oral rehydration solution improves thermoregulation in a mouse heatstroke model (2017)
  • Author(s): Kazuyuki Miyamoto, Hirokazu Ohtaki, Hiromi Takayasu, Atsuro Maeda, Jun Sasaki, Kazuho Honda, Munetaka Hayashi, Kenji Dohi
  • Journal Title: Critical Care Medicine Volume: 44 Pages: 402-402
• [Presentation] マウス熱中症モデルにおける経口補水液(OS-1)の有用性について (2018)
  • Author(s): 宮本和幸, 大滝博和, 髙安弘美, 前田敦雄, 佐々木純, 本田一穂, 土肥謙二, 林宗貴
  • Organizer: 第45回日本集中治療医学会学術集会
• [Presentation] 脳蘇生神経保護療法の開発を目指して ～基礎から臨床的検討、そして未来へ (2018)
  • Author(s): 土肥謙二
  • Organizer: 第64回昭和大学 学士会 総会
  • Invited
• [Presentation] マウス熱中症モデルにおける経口補水液の効果について (2017)
  • Author(s): 宮本和幸、大滝博和、高安弘美、前田敦雄、佐々木純、本多一穂、土肥謙二、林宗貴
  • Organizer: 第44回日本集中治療医学会総会・学術集会
  • Place of Presentation: 札幌市教育文化会館
  • Year and Date: 2017-03-10
• [Presentation] Oral rehydration solution improves thermoregulation in a mouse heatstroke model (2017)
  • Author(s): Kazuyuki Miyamoto, Hirokazu Ohtaki, Hiromi Takayasu, Atsuro Maeda, Jun Sasaki, Kazuho Honda, Munetaka Hayashi, Kenji Dohi
  • Organizer: 46th Critical Care Medicine
  • Place of Presentation: Hawaii, Honolulu, USA
  • Year and Date: 2017-01-21
  • Int'l Joint Research
• [Presentation] Production of MCP-1 (CCL2) from human mesenchymal stem/stromal cells in ischemic brain (2017)
  • Author(s): 2.Hirokazu Ohtaki, Kazumichi Yagura, Yuki Tanaka, Jun Watanabe, Kazuyuki Miyamoto, Yutaka Hiraizumi, Kenji Dohi, Kazuho Honda
  • Organizer: 第40回日本神経科学大会（幕張メッセ，千葉）
• [Presentation] Suppression of nitric oxide level from microglia by co-culturing with human mesenchymal stem/progenitor cells (2017)
  • Author(s): 8.Ohtaki H, Watanabe J, Yagura K, Miyamoto K, Hiraizumi Y, Honda K, Dohi K
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Therapeutic time-window for edaravone treatment of traumatic brain injury in mice. (2017)
  • Author(s): Miyamoto Kazuyuki, Ohtaki Hirokazu, Dohi, Kenji, Sitho Hiromi, Maeda Atsuo, Sasaki Jun, Honda Kazuho, Hayashi Munetaka
  • Organizer: 8th Joint Meeting of Society for Free Radical Research Australasia and Japan with International Symposium on Coenzyme Q10
  • Int'l Joint Research
• [Presentation] Human recombinant thrombomodulin (rTM) improves the neurological function in a murine heatstroke model (2016)
  • Author(s): Kazuyuki Miyamoto
  • Organizer: 45th Society of Critical Care Medicine
  • Place of Presentation: Orland, USA
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Book] Neurocritical care (2019)
  • Author(s): Dohi kenji
  • Publisher: springer