Roles of fatty acids and fatty acid-binding proteins (FABP) in cartilage resorption

• Project/Area Number: 26462796
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Meikai University
• Principal Investigator: AMANO Osamu 明海大学, 歯学部, 教授 (60193025)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂肪酸結合タンパク / 軟骨吸収 / 軟骨吸収細胞 / メッケル軟骨 / 骨端板 / セプトクラスト / 咽頭喉頭筋 / エネルギー代謝 / 脂肪酸結合蛋白 / 破骨細胞 / 石灰化

Outline of Final Research Achievements

We found the expression and localization of epidermal type-fatty acid-binding protein (EFABP) in septoclasts at the osseo-chondral junction of the growth plate in mice. Morphology of the septoclastic process was associated with an activity of the endochondral ossification. As E-FABP has affinity to retinoic acid, septoclasts showed apoptosis or morphological changes via PPAR and CD-RAP against overdose or deficiency of retinoic acid. E-FABP-positive septoclasts were also involved in absorption of the Meckel's cartilage.
This project reveled that septoclasts play important roles in physiological cartilage resorption and they are regulated by signaling pathways via E-FABP-binding fatty acids or retinoic acid.

Research Products

• [Journal Article] Induction of apoptosis in human oral keratinocyte by doxorubicin. (2017)
  • Author(s): Sakagami H, Okudaira N, Masuda Y, Amano O, Yokose S, Kanda Y, Suguro M, Natori T, Oizumi H, Oizumi T.
  • Journal Title: Anticancer Res Volume: 37 Pages: 1023-1029
  • Peer Reviewed / Open Access
• [Journal Article] Morphological Changes of Myoepithelial Cells in the Rat Submandibular Gland Following the Application of Surgical Stimuli (2016)
  • Author(s): Kawabe Y, Mizobe K, Bando Y, Sakiyama K, Taira F, Tomomura A, Araki H, Amano O
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 49 Issue: 6 Pages: 159-169
  • DOI: 10.1267/ahc.16017
  • NAID: 130006856399
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fetal Tendinous Connection Between the Tensor Tympani and Tensor Veli Palatini Muscles: A Single Digastric Muscle Acting for Morphogenesis of the Cranial Base. (2016)
  • Author(s): Rodríguez-Vázquez JF, Sakiyama K, Abe H, Amano O, Murakami G.
  • Journal Title: Anat Rec Volume: 299 Issue: 4 Pages: 474-483
  • DOI: 10.1002/ar.23310
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 第三の手・舌 (2016)
  • Author(s): 天野 修
  • Journal Title: 小児保健研究 Volume: 76 Pages: 706-710
  • Peer Reviewed / Open Access
• [Journal Article] 舌の不思議 (2016)
  • Author(s): 天野 修，坂東康彦，﨑山浩司
  • Journal Title: 小児歯科臨床 Volume: 21 Pages: 18-26
  • Peer Reviewed / Open Access
• [Journal Article] Development and regression of the thyroglossal duct in mice (2015)
  • Author(s): noue, K Bando, Y Sakiyama, K Takizawa, S Sakashita, H Kondo, H Amano, O
  • Journal Title: Annals of Anatomy Volume: 200 Pages: 54-65
  • DOI: 10.1016/j.aanat.2015.01.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] マウス咽頭・喉頭筋における心筋型脂肪酸結合タンパクの局在 (2015)
  • Author(s): 横塚裕二，重松久夫，坂東康彦，﨑山浩司，天野修，坂下英明
  • Journal Title: 小児口腔外科 Volume: 25 Pages: 126-139
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Localization and roles of hepatocyte growth factor (HGF) in development and disappearance of mouse Meckel's cartilage (2015)
  • Author(s): Tokunaga, H. Bando, Y. Sakiyama, K. Amano, O.
  • Journal Title: Journal of Meikai Dental Medicine Volume: 44 Pages: 74-86
  • Peer Reviewed
• [Journal Article] Immunolocalization of heat shock protein 25 (Hsp25) in developing secondary palate of mouse embryos (2014)
  • Author(s): Yamada, T Sasaki, A Bando, Y Sakiyama, K Suda, N Kanegae, H Amano, O
  • Journal Title: Pediatric Oral and Maxillofacial Surgery Volume: 24 Pages: 23-37
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Localization of Hsp27 in the Rat Submandibular Gland Following the Application of Various Surgical Treatments (2014)
  • Author(s): Mizobe, Kenichi Kawabe, Yoshihiro Bando, Yasuhiko Sakiyama, Koji Araki, Hisao Amano, Osamu
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 47 Issue: 6 Pages: 255-264
  • DOI: 10.1267/ahc.14037
  • NAID: 130004703746
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression of epidermal fatty acid binding protein (E-FABP) in septoclasts in the growth plate cartilage of mice. (2014)
  • Author(s): Bando, Y., Yamamoto, M., Sakiyama, K., Inoue, K., Takizawa, S., Owada, Y., Iseki, S., Kondo, H. and Amano, O.
  • Journal Title: J Mol Histol Volume: 45 Issue: 5 Pages: 507-518
  • DOI: 10.1007/s10735-014-9576-1
  • Peer Reviewed / Open Access
• [Presentation] マウス胎仔Meckel軟骨における低酸素誘導因子(HIF-1α)の局在と低酸素器官培養の影響 (2016)
  • Author(s): 坂下 英、坂東康彦、崎山浩司、坂下英明、天野 修
  • Organizer: 第53回日本口腔組織培養学会学術大会
  • Place of Presentation: 金沢市
  • Year and Date: 2016-11-17
• [Presentation] 解剖学者メッケル一族とメッケル軟骨 (2016)
  • Author(s): 天野 修
  • Organizer: 第44 回日本歯科医史学会学術大会
  • Place of Presentation: 東京都
  • Year and Date: 2016-10-01
  • Invited
• [Presentation] マウスメッケル軟骨におけるセプトクラストの局在 (2016)
  • Author(s): 坂下 英，坂東康彦，崎山浩司，天野 修
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] レチノイン酸過剰・欠乏における septoclastの形態変化と増殖抑制のメカニズム (2016)
  • Author(s): 坂東康彦，坂下 英，崎山浩司，天野 修
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2016-08-24
• [Presentation] 第三の手・舌 (2016)
  • Author(s): 天野 修
  • Organizer: 第63回日本小児保健協会学術集会
  • Place of Presentation: さいたま市
  • Year and Date: 2016-06-23
  • Invited
• [Presentation] 外分泌腺における筋上皮の意義ー唾液腺筋上皮の形態変化ー (2016)
  • Author(s): 天野 修，川邉好弘，溝部健一，平良芙蓉子，三宅言輝，﨑山浩司，荒木久夫
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま，福島県郡山市
  • Year and Date: 2016-03-28
  • Invited
• [Presentation] マウスメッケル軟骨におけるセプトクラストの分布と形態変化 (2016)
  • Author(s): 坂下 英，坂東康彦，﨑山浩司，大和田祐二，天野 修
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま，福島県郡山市
  • Year and Date: 2016-03-28
• [Presentation] マウス骨端板のseptoclastにおけるレチノイン酸欠乏，過剰による増殖抑制と形態変化 (2016)
  • Author(s): 坂東康彦，坂下 英，﨑山浩司，山本美由紀，井関尚一，大和田祐二，天野 修
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま，福島県郡山市
  • Year and Date: 2016-03-28
• [Presentation] ラットEbner腺の筋上皮細胞の分布と形態 (2016)
  • Author(s): 平良芙蓉子，川邉好弘，坂東康彦，﨑山浩司，天野 修
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま，福島県郡山市
  • Year and Date: 2016-03-28
• [Presentation] ラット舌下腺筋上皮におけるアディポネクチンの特異的局在 (2016)
  • Author(s): 三宅言輝，平良芙蓉子，坂東康彦，﨑山浩司，天野 修
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビックパレットふくしま，福島県郡山市
  • Year and Date: 2016-03-28
• [Presentation] 癌が舌筋の形態や特性に与える影響 (2015)
  • Author(s): 﨑山浩司，瀧澤将太，坂東 康彦，坂下 英，天野 修
  • Organizer: 第57回歯科基礎医学会学術大会・総会
  • Place of Presentation: 朱鷺メッセ，新潟県新潟市
  • Year and Date: 2015-09-11
  • Invited
• [Presentation] マウスメッケル軟骨におけるセプトクラストの局在と三次元的形態 (2015)
  • Author(s): 坂下 英，坂東康彦，平良芙蓉子，﨑山浩司，天野 修
  • Organizer: 第57回歯科基礎医学会学術大会・総会
  • Place of Presentation: 朱鷺メッセ，新潟県新潟市
  • Year and Date: 2015-09-11
• [Presentation] レチノイン酸欠乏・過剰による septoclast の増殖抑制と形態変化の誘導 (2015)
  • Author(s): 坂東康彦，坂下 英，﨑山 浩司，天野 修
  • Organizer: 第57回歯科基礎医学会学術大会・総会
  • Place of Presentation: 朱鷺メッセ，新潟県新潟市
  • Year and Date: 2015-09-11
• [Presentation] Influence by excess and deficiency of retinoid acids on septoclasts in the epiphyseal plate of mice (2015)
  • Author(s): Bando Y, Sakashita H, Kawabe Y, Sakiyama K, Yamamoto M, Iseki S, Owada Y, Amano O.
  • Organizer: The 120th Annual Meeting of the Japanese Association of Anatomists and the 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kobe
  • Year and Date: 2015-03-21 – 2015-03-23