| Project/Area Number |
26462810
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
Nishida Takashi 岡山大学, 医歯薬学総合研究科, 准教授 (30322233)
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| Co-Investigator(Kenkyū-buntansha) |
滝川 正春 岡山大学, 医歯薬学総合研究科, 教授 (20112063)
久保田 聡 岡山大学, 医歯薬学総合研究科, 教授 (90221936)
服部 高子 岡山大学, 医歯薬学総合研究科, 助教 (00228488)
青山 絵理子 岡山大学, 医歯薬学総合研究科, 助教 (10432650)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | CCN2/CTGF / 骨細胞様細胞 / 破骨細胞形成 / タモキシフェン誘導性Ccn2欠損マウス / 3次元培養 / 老齢期のタモキシフェン誘導性CCN2欠損マウス / NFATc1 / カテプシンK / 骨細胞 / 初代骨細胞様細胞 / 薬剤誘導性Ccn2欠損マウス / マウス骨細胞株MLO-Y4 |
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| Outline of Final Research Achievements |
It is well-known that osteocytes play a role in the regulation of bone remodeling. However, the effect of CCN2/CTGF on the bone remodeling in osteocytes is unknown. Therefore, the aim of this study is the investigation of osteoclastogenesis via osteocytes stimulated by CCN2. A mouse osteocytic cell line, MLO-Y4 embedded into collagen gel containing recombinant CCN2 protein, promoted the osteoclast differentiation of RAW264.7 cells inoculated on the collagen gel, and osteocyte-like cells isolated from Ccn2-deficient mice inhibited the differentiation of osteoclasts under the same condition. These findings suggest that osteoclastogenesis is modulated by osteocytes stimulated by CCN2.
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