Regulation of bone remodeling by Annexin A5 at tendon/ligament insertion sites
Project/Area Number |
26462825
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional basic dentistry
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Research Institution | Tsurumi University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
二藤 彰 鶴見大学, 歯学部, 教授 (00240747)
和田 悟史 鶴見大学, 歯学部, 助教 (20581119)
山下 照仁 松本歯科大学, 総合歯科医学研究所, 准教授 (90302893)
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Research Collaborator |
ARAI Yoshinori
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | Annexin a5 / 腱 / 靭帯 / 骨 / アネキシンA5 |
Outline of Final Research Achievements |
Entheses can be further divided to fibrocartilaginous and fibrous entheses according to the type of tissue present at the skeletal attachment site. In Anxa5-deficient (Anxa5-/-) mice, the sizes of bone ridge outgrowths at the fibrocartilaginous entheses of tibiae and femur were increased after 7 weeks of age. This bone overgrowth was not observed at the fibrous enthesis where Anxa5-lacZ is hardly expressed. At the fibrocartilaginous entheses, more ALP-expressing cells were observed in the fibrocartilage layer outside of the bone in the mutant mice. Then, to examine the role of Anxa5 on cartilage differentiation, we performed Anxa5 gene knockdown in vitro using siRNA. Depletion of endogenous Anxa5 with siRNA increased mRNA expressions of differentiated marker of chondrocyte. Together, these results suggest that Anxa5 prevents bone overgrowth at the fibrocartilaginous entheses.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization.2015
Author(s)
Kanemoto S, Kobayashi Y, Yamashita T, Miyamoto T, Cui M, Asada R, Cui X, Hino K, Kaneko M, Takai T, Matsuhisa K, Takahashi N, Imaizumi K.
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Journal Title
J Cell Sci
Volume: 128
Pages: 4353-4365
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Noncanonical Wnt5a enhances Wnt/-catenin signaling during osteogenesis.2014
Author(s)
Okamoto, M., Udagawa, N., Yamashita, T., Nakamichi, Y., Uehara, S., Kato, H., Saito, N., Minami, Y., Takahashi, N., Kobayashi, Y.
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Journal Title
Sci. report
Volume: 4
Issue: 1
Pages: 4493-4493
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Histone methyltransferase G9a is essential to progress osteoblastic differentiation in vitro, and skull bone formation in vivo.2015
Author(s)
Ideno H, Komatsu K, Shimada A, Kamiunten T, Arai Y, Nakashima K, Araki R, Nakamura Y, Abe M, Tachibana M, Kimura H, Nifuji A.
Organizer
ASCB annual meeting
Place of Presentation
San Diego Convention Center (USA, CA, San Diego)
Year and Date
2015-12-12
Related Report
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[Presentation] Deletion of G9a, histone methyltransferase, causes impaired bone formation.2015
Author(s)
Ideno H, Komatsu K, Shimada A, Kamiunten T, Arai Y, Nakashima K, Araki R, Nakamura Y, Abe M, Tachibana M, Kimura H, Nifuji A
Organizer
Joint ANZBMS, MEPSA and MBSANZ Annual Scientific Meeting
Place of Presentation
Hotel Grand Chancellor Hobart (Australia, Tasmania)
Year and Date
2015-11-01
Related Report
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[Presentation] Annexin A5 inhibits bony outgrowth at tendon/ligament insertion sites.2015
Author(s)
Shimada A, Arai Y, Wada S, Ideno H, Kamiunten T, Nakashima K, Komatsu K, Yamashita T, Ezura Y, Amizuka N, Pöschl E, Brachvogel B, Nakamura Y, Nifuji A
Organizer
ASBMR 2015 annual meeting
Place of Presentation
Washington State Convention Center (USA, WA, Seattle)
Year and Date
2015-10-09
Related Report
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[Presentation] Histone 3 lysine 9 methyltransferase G9a is essential for the growth and differentiation of tenocytes.2015
Author(s)
Wada S, Ideno H, Shimada H, Kamiunten T, Nakamura Y, Nakashima K, Kimura H, Tachibana M, Nifuji A.
Organizer
ASBMR 2015 annual meeting
Place of Presentation
Washington State Convention Center (USA, WA, Seattle)
Year and Date
2015-10-09
Related Report
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