| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In a previous study, I investigated samples of culture supernatants using a combination of mass spectrometry and single immunohistochemistry. This method identified some proteins expressed in bovine periosteum-derived cells. In this study, I investigated the interactions of these proteins.Double immunohistochemistry was carried out to reveal interactions of type 1 procollagen and other proteins. The results showed that type 1 procollagen overlapped with TMX2. Therefore, type 1 procollagen and TMX2 may interact with each other.
FBXW2 (F-box/WD repeat-containing protein 2) was found in the periosteum, while other proteins were expressed in periosteum-derived cells., Mass spectrometry was carried out using cytoplasmic extracts. Immunohistochemistry of candidate protein ARHGAP36 revealed that this protein was not always expressed in periosteum-derived cells.
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