Identification of cancer stem cell niche in oral cancer and their therapeutic application
Project/Area Number |
26463035
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Hokkaido University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
間石 奈湖 北海道大学, 遺伝子病制御研究所, 助教 (00632423)
秋山 廣輔 北海道大学, 遺伝子病制御研究所, 客員研究員 (10609100)
樋田 泰浩 北海道大学, 大学病院, 准教授 (30399919)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | がん幹細胞 / 腫瘍血管 |
Outline of Final Research Achievements |
In this study, we investigated the role of tumor endothelial cells (TECs) for maintenance of cancer cell stemness. We analyzed the crosstalk of cancer stem cells (CSCs) and endothelial cells in vitro. Treatment of cultured conditioned medium of CSC-enriched cells increased the expression of TEC markers in endothelial cells. We next analyzed the signal pathways after co-culture of cancer cells with TECs. NF-kB and ERK1/2 pathways were activated in cancer cells after co-culture with high grade tumor derived TECs. In addition, colony formation was observed by co-culture with cancer cells and TECs. These data suggested that TEC-expressing molecules are involved in maintenance of stemness in cancer cells.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Vasohibin-1 as a novel prognostic factor for head and neck squamous cell carcinoma2017
Author(s)
Torii C., Hida Y., Shindoh M., Akiyama K., Ohga N., Maishi N., Ohiro Y., Ono M., Totsuka Y., Kitagawa Y., Tei K., Sato Y., Hida K.
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Journal Title
Anticancer Res
Volume: 37
Pages: 1219-1225
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Aneuploidy of murine immortalized endothelial cell line, MS12017
Author(s)
Hida K., Maishi N., Dorcas Akuba-Muhyia Annan, Kondoh M., Hojo T., Umma Habiba, Ohga N., Ishikawa K, Sato M., Torii C., Yanagiya M., Morimoto M., Hida Y., Shindoh M.
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Journal Title
J Oral Biosci
Volume: 59
Pages: 50-54
Related Report
Peer Reviewed
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[Journal Article] Tumor endothelial cells express high pentraxin 3 levels2016
Author(s)
Hida K., Maishi N., Kawamoto T., Akiyama K., Ohga N., Hida Y., Yamada K., Hojo T., Kikuchi H., Sato M., Torii C., Shinohara N., Shindoh M
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Journal Title
Pathol Int
Volume: 66
Pages: 687-694
DOI
Related Report
Peer Reviewed
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[Journal Article] Tumour endothelial cells in high metastatic tumours promote metastasis via epigenetic dysregulation of biglycan.2016
Author(s)
5.Maishi N., Ohba Y., Akiyama K., Ohga N., Hamada J., Nagao-Kitamoto H., Mohammad Towfik Alam, Yamamoto K., Kawamoto T., Inoue N., Taketomi A., Shindoh M., Hida Y., *Hida K.
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Journal Title
Sci Rep.
Volume: 6
Pages: 28039
DOI
Related Report
Peer Reviewed
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[Journal Article] Inhibition of multidrug transporter in tumor endothelial cells enhances antiangiogenic effects of low-dose metronomic paclitaxel2015
Author(s)
Akiyama K., Maishi N., Ohga N., Hida Y., Ohba Y., Alam Mohammad Towfik, Kawamoto T., Ohmura H., Yamada K., Torii C., Shindoh M. and *Hida K
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Journal Title
Am J Pathol
Volume: 185(2)
Pages: 572-580
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Inhibition of multidrug transporter in tumor endothelial cells enhances antiangiogenic effects of low-dose metronomic paclitaxel2014
Author(s)
Akiyama K., Maishi N., Ohga N., Hida Y., Ohba Y., Alam Mohammad Towfik, Kawamoto T., Ohmura H., Yamada K., Torii C., Shindoh M. and*Hida K.
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Journal Title
Am J Pathol
Volume: 185(2)
Pages: 572-580
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of Tumor Endothelial Cells with High Aldehyde Dehydrogenase Activity and a Highly Angiogenic Phenotype2014
Author(s)
Ohmura-Kakutani H., Akiyama K., Maishi N., Ohga N., Hida Y., Kawamoto T., Iida J., Shindoh M., Tsuchiya K., Shinohara N., *Hida K
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Journal Title
PLoS ONE
Volume: 9(12)
Pages: e113910
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of Novel Targets for Antiangiogenic Therapy by Comparing the Gene Expressions of Tumor and Normal Endothelial Cells2014
Author(s)
Otsubo T., Hida Y., Ohga N., Sato H., Kai T., Matsuki Y., Takasu H., Akiyama K., Maishi N., Kawamoto T., Shinohara N., Nonomura K., *Hida K
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Journal Title
Cancer Sci
Volume: 105(5)
Pages: 560-567
DOI
Related Report
Peer Reviewed
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[Presentation] Tumor endothelial cells acquire drug resistance by exosomal-miR derived from high metastatic tumor2016
Author(s)
3.Hida K., Torii C., Maishi N., Morimoto M., Akiyama K., Kawamoto T., Minami T., Yoshioka Y., Mohammad Towfik Alam, Shindoh M., Hida Y., Ochiya T
Organizer
The 19th International Vascular Biology Meeting
Place of Presentation
Boston(America)
Year and Date
2016-11-02
Related Report
Int'l Joint Research
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