Analysis of mouse behavior under different gravity conditions

• Project/Area Number: 26506031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: Japan Aerospace EXploration Agency
• Principal Investigator: Shiba Dai 国立研究開発法人宇宙航空研究開発機構, 有人宇宙技術部門, 主任研究開発員 (50360722)
• Co-Investigator(Renkei-kenkyūsha): MORITA hironobu 岐阜大学, 医学研究科, 教授 (80145044) ; SUGIYAMA noriyuki 大阪医科大学, 医学部, 講師 (90381954)
• Research Collaborator: SHIMOMURA michihiko 国立研究開発法人宇宙航空研究開発機構, 有人宇宙技術部門, 主任研究開発員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 重力 / 前庭機能 / 平衡機能 / 行動解析 / センサー / 過重力 / ストレス / 定量化 / 重力変化 / 一次繊毛 / 重力ストレス / 行動観察 / 受容体

Outline of Final Research Achievements

Animals have a function to sense acceleration changes in the inner ear, but it is not clear at the molecular level about how much changes can be detected and how to sense the subtle changes. In addition, while animals have a system to sense changes, they can adapt to a new environment if the change is in a permanent state. In other words, the sensing system is sensitive and flexible.
In this study, in order to analyze the activity under the hyper gravity environment, the cage with video camera, which can be housed individually, was placed in a centrifuge and the mice were reared for 2 weeks. In addition, we developed a "pixel analysis system" that analyzes the activity from mouse behavior, and evaluated the time when the mice were mostly affected by hyper gravity.

Research Products

• [Journal Article] Impact of a simulated gravity load for atmospheric reentry, 10 g for 2 min, on conscious mice. (2017)
  • Author(s): Morita H, Yamaguchi A, Shiba D, Shirakawa M, Takahashi S.
  • Journal Title: J Physiol Sci. Volume: 印刷中
  • NAID: 40021238561
  • Peer Reviewed
• [Journal Article] Effects of gravity changes on gene expression of BDNF and serotonin receptors in the mouse brain (2017)
  • Author(s): Chihiro Ishikawa, Haiyan Li, Rin Ogura, Yuko Yoshimura, Takashi Kudo, Masaki Shirakawa, Dai Shiba, Satoru Takahashi, Hironobu Morita and Takashi Shiga
  • Journal Title: Plos One Volume: 印刷中
  • NAID: 120007128995
  • Peer Reviewed / Open Access
• [Journal Article] Feasibility of a Short-arm Centrifuge for Mouse Hypergravity Experiments (2015)
  • Author(s): Morita H, Obata K, Abe C, Shiba D, Shirakawa M, Kudo T, Takahashi T
  • Journal Title: PLoS ONE Volume: 10-7
  • NAID: 120007136296
  • Peer Reviewed / Open Access
• [Journal Article] Hypergravity Provokes a Temporary Reduction in CD4+CD8+ Thymocyte Number and a Persistent Decrease in Medullary Thymic Epithelial Cell Frequency in Mice (2015)
  • Author(s): Tateishi R, Akiyama N, Miyauchi M, Yoshinaga R, Sasanuma H, Kudo T, Shimbo M, Shinohara M, Obata K, Inoue J, Shirakawa M, Shiba D, Asahara H, Yoshida N, Takahashi S, Morita H, Akiyama T
  • Journal Title: PLoS ONE Volume: 10-10
  • Peer Reviewed / Open Access
• [Presentation] Analysis of mouse behavior under different gravity conditions (2015)
  • Author(s): Dai Shiba
  • Organizer: International Society for ADAPTIVE MEDICINE (ISAM 2015)
  • Place of Presentation: YONAGO CONVENTION CENTER
  • Year and Date: 2015-05-27 – 2015-05-30
  • Invited
• [Presentation] Analysis of mouse behavior under different gravity conditions (2015)
  • Author(s): Dai Shiba
  • Organizer: 11th World Congress of the International Society for Adaptive Medicine
  • Place of Presentation: Yonago, Japan
  • Year and Date: 2015-05-27
  • Int'l Joint Research / Invited