The model for carbon cycle in the mangrove based on the cooperative degradation of cellulose by the crabs and bacteria

• Project/Area Number: 26520308
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Food Cycle Research
• Research Institution: Kochi University
• Principal Investigator: ADACHI KOHSUKE 高知大学, 教育研究部自然科学系農学部門, 准教授 (00399114)
• Co-Investigator(Kenkyū-buntansha): 池島 耕 高知大学, 教育研究部自然科学系農学部門, 教授 (30582473)
• Project Period (FY): 2014-07-18 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: mangrove / mangrove crabs / material cycle / cellulose degradation / intestinal bacteria / soil bacteria / マングローブ / 炭素循環 / カニ / 土壌 / 腸内細菌 / セルロース分解 / トラン県シカオ / 土壌細菌

Outline of Final Research Achievements

In this study we investigated the relation between the litter and mangrove crabs which plays crucial roles in mangrove material flow. From the results of litter trap analysis, the behavior observation of the crabs by video camera, and their breeding experiment, it was calculated that 34% of the litter feed the crabs in the experimental field. In the metagenome analysis, proteobacteria was predominant in both intestinal soil bacteria. Some of the OUT was assigned in “cellulose degradation” in the phenotype analysis of metagenassist (http://www.metagenassist.ca/METAGENassist/faces/Home.jsp). Cellulose degrading activity was found in the both intestinal soil bacteria of which value was　about 10- 100mg/kg soil/24h in Deng 1994 method. Taken together with our previous results, it was indicated that the cellulose in about 1/3 of the litter was sequentially digested by three factors: digestive enzyme, intestinal bacteria and soil bacteria.

Research Products

• [Journal Article] Cellulolytic activity in the hepatopancreas of Chionoecetes opilio and Chionoecetes japonicus: enzymatic adaptations to deep sea environment, (2016)
  • Author(s): Adachi, K., Tanimura, K., Mitsui, T., Morita, T., Yosho, I., Ikejima, K., Morioka, K.
  • Journal Title: Fisheries Science, Volume: 82 Issue: 5 Pages: 835-841
  • DOI: 10.1007/s12562-016-1014-8
  • NAID: 40020941597
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] マングローブクラブ腸内細菌とその生息土壌の持つセルロース分解活性 (2016)
  • Author(s): 足立亨介, 杉岡茉美, Prasert Tongnunui, 荒木通啓, 森岡克司, 池島耕.
  • Organizer: 平成28年度 第22回日本マングローブ学会大会
  • Place of Presentation: 於 東京農業大学、日本
  • Year and Date: 2016-12-10
• [Presentation] ビデオ観察によるマングローブ林のベンケイガニ類生息密度の推定. (2016)
  • Author(s): 鈴木実央, 池島 耕, 足立亨介, Prasert Tongnunui.
  • Organizer: 平成28年度 第22回日本マングローブ学会大会
  • Place of Presentation: 於 東京農業大学、日本
  • Year and Date: 2016-12-10
  • Int'l Joint Research
• [Presentation] RNA seq analysis of hepatopancreas of mangrove crab Episesarma versicolor (2016)
  • Author(s): Kohsuke Adachi, Kohei Kawai, Michihiro Araki, Prasert Tongnunui, Katsuji Morioka, Kou Ikejima.
  • Organizer: International Conference in Challenges in Environmental Science & Engineering
  • Place of Presentation: Kaohsiung, Taiwan
  • Year and Date: 2016-11-06
  • Int'l Joint Research
• [Presentation] マングローブ炭素循環におけるカニの役割 (2015)
  • Author(s): 足立亨介
  • Organizer: 平成26年度高知大学自然科学系プロジェクト「水・バイオマス」公開シンポジウム
  • Place of Presentation: 高知大学農学部
  • Year and Date: 2015-03-10
• [Presentation] 目視とビデオ観察によるマング ローブ林のベンケイガニ類の生息密度推定の試み (2014)
  • Author(s): 鈴木実央・池島 耕・足立亨介・P.Tongnunui
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学
  • Year and Date: 2014-09-19 – 2014-09-22