| Project/Area Number |
26560398
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SHIUCHI Tetsuya 徳島大学, 大学院医歯薬学研究部, 准教授 (70372729)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 運動 / 視床下部 / レプチン / レプチン感受性 / オレキシン |
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| Outline of Final Research Achievements |
In this study, leptin sensitivity in hypothalamus after endurance exercise and its mechanism were examined. Male C57BL/6J mice were performed treadmill running for 45 minutes. After that, leptin was injected intraperitoneally. Forty-five minutes later, hypothalamus in mice was collected, and determined the phosphorylation of STAT3 by western blot or immunohistochemistry.
Treadmill running enhanced STAT3 phosphorylation in hypothalamus of the mice by ip injection of leptin. Immunohistochemical staining revealed that treadmill running increased c-Fos expression in one neuropeptidergic neuron in hypothalamus of the mice. Intracelebroventricular injection of receptor antagonist of the neuropeptide inhibited the exercise-induced enhancement of STAT3 phosphorylation in hypothalamus by leptin. Taken together, it is suggested that the enhanced leptin sensitivity after exercise is mediated by activation of the neuropeptidergic neuron by exercise.
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