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Elucidation of physical properties and chemical components of glue secretion used in breeding behavior of Breviceps frog and attempt at its artificial breeding

Research Project

Project/Area Number 26560437
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomolecular chemistry
Research InstitutionHiroshima University

Principal Investigator

Kurabayashi Atsushi  広島大学, 両生類研究センター, 助教 (00327701)

Co-Investigator(Kenkyū-buntansha) 住田 正幸  広島大学, 理学研究科, 教授 (10163057)
広瀬 裕一  琉球大学, 理学部, 教授 (30241772)
Co-Investigator(Renkei-kenkyūsha) UKENA Kazuyoshi  広島大学, 総合科学研究科, 教授 (10304370)
Research Collaborator SAWADA Hitoshi  
NAKAZAWA Shiori  
HEMMI Keitaro  
VENCES Miguel  
MALONE John  
MINTER Leslie  
Du PREEZ Louis  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords生体機能物質 / 非モデル動物における生体物質の探索 / 人工繁殖 / 糊状物質 / プロテオーム / トランスクリプトーム / 接着物質 / mRNA-Seq / 生体機能分子 / フクラガエル / フクラガエル属 / 種の保全 / 生体物質 / 生化学 / 国際情報交換_ドイツ_アメリカ_南アフリカ / プロテオーム解析 / トランスクリプトーム解析
Outline of Final Research Achievements

We investigated the physical properties, chemical components, and candidate genes of the glue secration used in the reproduction activity in Breviceps frogs. As the results of this study, the adhesion strength of glue secration was about 500 g/cm2, and it was found that its main constituent is protein. It was suggested that there are four candidate proteins of the frog glue: three proteins similar to a non-glue skin-secrated protein known in another frog taxon and one novel protein.
We attempted at the artificial breeding of B. adspersus. As the result, it was revealed that a cactail of gonadotropin-releasing hormone agonist and dopamine antagonist, called Amphiplex, can induce the ovulation of this species. In addition, we succeeded to artificailly induce their amphiplexus and observe the nesting and spwning under the captive condition for the first time.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2016 Other

All Int'l Joint Research (4 results) Presentation (1 results)

  • [Int'l Joint Research] コネチカット大学(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] ノースウェスト大学(南アフリカ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] ノース・ウェスト大学/リンポポ大学(南アフリカ)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] コネチカット大学(米国)

    • Related Report
      2015 Research-status Report
  • [Presentation] フクラガエルの「糊」と「人工繁殖」と「ミトコンドリアゲノム」に関する新知見2016

    • Author(s)
      逸見敬太郎・掛橋竜祐・John Malone・Louis Du Preez・Leslie Minter・倉林敦
    • Organizer
      日本爬虫両棲類学会第55回年次大会
    • Place of Presentation
      琉球大学, 沖縄県
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2022-02-16  

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