Development of fluorescence-emitting photoaffinity probe that enables the visualization of cross-linking process within cells.
| Project/Area Number |
26560449
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NAMBA Kosuke 徳島大学, 大学院医歯薬学研究部, 教授 (50414123)
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| Co-Investigator(Renkei-kenkyūsha) |
MURATA Yoshiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主席研究員 (60256047)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 蛍光分子 / 光親和性標識 / チオールセンサー / 単分子白色発光 / 1,3a,6a-トリアザペンタレン / 蛍光標識 / ケミカルバイオロジー / 蛍光プローブ / 白色蛍光 / チオール標識 / システイン標識 / グルタチオン標識 |
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| Outline of Final Research Achievements |
A white-light-emitting molecule was developed. Since the fluorescence color of this molecule was changed from white to blue by UV irradiation within several minutes, this molecule was suggested to be a useful photoaffinity probe that can detect the labeling process of target protein by the observation of fluorescence color change. Furthermore, development of yellow and red fluorescence compact molecules and novel thiol sensor, substantially increasing the fluorescence intensity by the specific addition of thiol groups, were achieved.
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Report
(3 results)
Research Products
(89 results)
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[Journal Article] Total Synthesis of Palau’amine2015
Author(s)
K. Namba, K. Takeuchi, Y. Kaihara, M. Oda, A. Nakayama, A. Nakayama, M. Yoshida, and K. Tanino
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Journal Title
Nature Communications
Volume: 6
Issue: 1
Pages: 8731-8731
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Synthesis of yellow and red fluorescent 1,3a,6a-triazapentalenes and the theoretical investigation of their optical properties2015
Author(s)
Kosuke Namba, Ayumi Osawa, Akira Nakayama, Akane Mera, Fumi Tano, Yoshiro Chuman, Eri Sakuda, Tetsuya Taketsugu, Kazuyasu Sakaguchi, Noboru Kitamura, Keiji Tanino
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Journal Title
Chemical Science
Volume: 6
Issue: 2
Pages: 1083-1093
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] パラウアミンの全合成2015
Author(s)
竹内公平、海原由香理、難波康祐、谷野圭持
Organizer
日本化学会第95年会
Place of Presentation
日本大学（千葉県船橋市）
Year and Date
2015-03-26
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