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In vitro fabrication of bone-like structures for tissue engineering applications

Research Project

Project/Area Number 26630423
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionYokohama National University

Principal Investigator

Fukuda Junji  横浜国立大学, 工学(系)研究科(研究院), 准教授 (80431675)

Co-Investigator(Kenkyū-buntansha) ANADA TAKAHISA  東北大学, 歯学研究科, 准教授 (30398466)
SUZUKI ATSUSHI  横浜国立大学, 工学研究院, 准教授 (60467058)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords再生医療 / 骨 / 血管 / コラーゲン / 間葉系幹細胞 / 骨組織
Outline of Final Research Achievements

Fabrication of three-dimensional vascularized bone graft is a major challenge in bone tissue engineering. A collagen gel solution containing bone marrow derived mesenchymal stem cells (MSCs) was dropped on a hydrophobic plate, which then spontaneously contracted and formed spherical shape during culture by contraction forces of MSCs. The surface of MSC-embedded microgel was covered with human umbilical vein endothelial cells. Transplantation of the microgels with endothelial cells into a bone-defeated model mouse revealed acceleration of bone regeneration with improved blood supply, compared to microgels without endothelial cells, clinically proven biomaterial (beta-TCP), and non-transplantation control. This approach may be a fundamental technique for engineering osteon-like tissues to bone regenerative medicine.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (51 results)

All 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (18 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 18 results,  Acknowledgement Compliant: 7 results,  Open Access: 2 results) Presentation (21 results) (of which Invited: 6 results) Book (7 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Stanford University(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] IRCCS Galeazzi Orthopaedic Institute(Italy)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] In situ cross-linkable gelatin-CMC hydrogels designed for rapid engineering of perfusable vasculatures2016

    • Author(s)
      T. Kageyama, T. Osaki, J. Enomoto, D. Myasnikova, T. Nittami, T. Hozumi, T. Ito, and J. Fukuda
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rational design of prevascularized large 3D tissue constructs using computational simulations and geometric control of self-assembled monolayers2016

    • Author(s)
      C. Arrigoni, M. Bongio, G. Talo, S. Bersini, J. Enomoto, J. Fukuda, M. Moretti
    • Journal Title

      Advanced healthcare materials

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An oxygen-permeable spheroid culture chip (Oxy chip) promotes osteoblastic differentiation of mesenchymal stem cells2016

    • Author(s)
      T. Kamoya, T. Anada, Y. Shiwaku, T. Takano-Yamamoto, O. Suzuki
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 232 Pages: 75-83

    • DOI

      10.1016/j.snb.2016.03.107

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Evaluation of bioactivity of octacalcium phosphate using osteoblastic cell aggregates on a spheroid culture device2016

    • Author(s)
      T. Anada, T. Sato, T. Kamoya, Y. Shiwaku, K. Tsutchiya, T. Takano-Yamamoto, K. Sasaki, O. Suzuki
    • Journal Title

      Regenerative Therapy

      Volume: 3 Pages: 58-62

    • DOI

      10.1016/j.reth.2016.02.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Oriented bone regenerative capacity of octacalcium phosphate/gelatin composites obtained through two-step crystal preparation method2016

    • Author(s)
      Ishiko-Uzuka R, Anada T, Kobayashi K, Kawai T, Tanuma Y, Sasaki K, Suzuki O
    • Journal Title

      J Biomed Mater Res B Appl Biomater

      Volume: in press Issue: 5 Pages: 1029-1039

    • DOI

      10.1002/jbm.b.33640

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative study on the resorbability and dissolution behavior of octacalcium phosphate, <i>β</i>-tricalcium phosphate, and hydroxyapatite under physiological conditions2016

    • Author(s)
      S Sakai, T Anada, K Tsuchiya, H Yamazaki, HC Margolis, O Suzuki.
    • Journal Title

      Dental Materials Journal

      Volume: 35 Issue: 2 Pages: 216-224

    • DOI

      10.4012/dmj.2015-255

    • NAID

      130005141804

    • ISSN
      0287-4547, 1881-1361
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development.2016

    • Author(s)
      Suzuki A, Niimi Y, Shinmyozu K, Zhou Z, Kiso M, Saga Y.
    • Journal Title

      EMBO Rep.

      Volume: 17(1) Issue: 1 Pages: 37-46

    • DOI

      10.15252/embr.201540828

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Engineering a vascularized collagen-β-tricalcium phosphate graft using an electrochemical approach2015

    • Author(s)
      Y. Kang, N. Mochizuki, A. Khademhosseini, J. Fukuda, Y. Yang
    • Journal Title

      Acta Biomaterialia

      Volume: 11 Pages: 449-458

    • DOI

      10.1016/j.actbio.2014.09.035

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Enhancement of osteoblastic differentiation in alginate gel beads with bioactive octacalcium phosphate particles.2015

    • Author(s)
      K Endo, T Anada, M Yamada, M Seki, K Sasaki, O Suzuki
    • Journal Title

      Biomed Mater

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Orthotopic osteogenecity enhanced by a porous gelatin sponge in a critical-sized rat calvaria defect.2015

    • Author(s)
      N Kanda, T Anada, T Handa, K Kobayashi, Y Ezoe, T Takahashi, O Suzuki
    • Journal Title

      Macromol Biosci,

      Volume: -

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate-gelatin scaffold2015

    • Author(s)
      M Yamada, T Anada, T Masuda, T Takano-Yamamoto, O Suzuki
    • Journal Title

      Sens Actuators B Chem

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chondroitin sulfate-E binds to both osteoactivin and integrin αVβ3 and inhibits osteoclast differentiation2015

    • Author(s)
      T Miyazaki, S Miyauchi, T Anada, A Tawada, O Suzuki
    • Journal Title

      J Cell Biochem

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biomechanical Evaluation of Ti-Nb-Sn Alloy Implants with a Low Young's Modulus.2015

    • Author(s)
      Takahashi K, Shiraishi N, Ishiko-Uzuka R, Anada T, Suzuki O, Masumoto H, Sasaki K.
    • Journal Title

      Int J Mol Sci.

      Volume: 16 Issue: 3 Pages: 5779-5788

    • DOI

      10.3390/ijms16035779

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The role of an octacalcium phosphate in the reformation of infraspinatus tendon insertion2015

    • Author(s)
      Itoigawa Y, Suzuki O, Sano H, Anada T, Handa T, Hatta T, Kuwahara Y, Takahashi A, Ezoe Y, Kaneko K, Itoi E.
    • Journal Title

      J Shoulder Elbow Surg

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of partially hydrolyzed OCP crystals deposited in a gelatin matrix as a scaffold for bone tissue engineering.2015

    • Author(s)
      Ezoe Y, Anada T, Yamazaki H, Handa T, Kobayashi K, Takahashi T, Suzuki O
    • Journal Title

      J Nanopart Res

      Volume: 17 Issue: 3 Pages: 140-140

    • DOI

      10.1007/s11051-015-2864-1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Acceleration of vascular sprouting from fabricated perfusable vascular-like structures2015

    • Author(s)
      T.Osaki, T. Kakegawa, T. Kageyama, J. Enomoto, T. Nittami and J. Fukuda,
    • Journal Title

      PLoS one

      Volume: 10(4) Issue: 4 Pages: e0123735-e0123735

    • DOI

      10.1371/journal.pone.0123735

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rapid engineering of cell-lined vascular-like structures in in situ crosslinkable hydrogels2014

    • Author(s)
      T. Kageyama, T. Kakegawa, T. Osaki, J. Enomoto, T. Ito, T. Nittami, J. Fukuda
    • Journal Title

      Biofabrication

      Volume: 6 Issue: 2 Pages: 25006-25006

    • DOI

      10.1088/1758-5082/6/2/025006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reverse transfection in microchamber arrays for cell migration assays2014

    • Author(s)
      J. Enomoto, R. Takagi, R. Onuki-Nagasaki, S. Fujita, and J. Fukuda
    • Journal Title

      Sensors & Actuators: B

      Volume: 190 Pages: 896-9

    • DOI

      10.1016/j.snb.2013.09.014

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 電気化学を用いた再生医療のための立体臓器の作製2016

    • Author(s)
      福田淳二
    • Organizer
      第2回理論応用力学シンポジウム
    • Place of Presentation
      日本学術会議講堂
    • Year and Date
      2016-03-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 細胞組織構築のための 細胞接着性の制御2015

    • Author(s)
      福田淳二
    • Organizer
      第25回日本MRS年次大会
    • Place of Presentation
      横浜市開港記念会館
    • Year and Date
      2015-12-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 材料表面の細胞接着制御 による細胞の操作2015

    • Author(s)
      福田淳二
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 細胞包埋石灰化ビーズ(Bone beads)を用いた血管導入骨組織の3次元構築2015

    • Author(s)
      園山由希江、景山達斗、福田淳二
    • Organizer
      第37回日本バイオマテリアル学会大会
    • Place of Presentation
      京都テルサ
    • Year and Date
      2015-11-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞包埋石灰化ビーズ(Bone beads)を用いた血管導入骨組織の構築2015

    • Author(s)
      園山由希江、景山達斗、福田淳二
    • Organizer
      第67回日本生物工学会
    • Place of Presentation
      城山観光ホテル(鹿児島)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Electrochemical cell detachment for engineering vascularized 3D tissues2015

    • Author(s)
      J. Fukuda
    • Organizer
      E-MRS 2015 (European Materials Research Society)
    • Place of Presentation
      Lille, France
    • Year and Date
      2015-05-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 射出成形技術を模した血管導入骨組織の構築2015

    • Author(s)
      景山達斗、園山由希江、吉村知紗、伊藤 大知、穴田貴久、鈴木治、福田 淳二
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 電気化学を用いた細胞操作2015

    • Author(s)
      福田淳二
    • Organizer
      公益社団法人電気化学会第82回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2015-03-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 電気化学細胞脱離を用いた血管導入骨組織の構築2015

    • Author(s)
      景山達斗、掛川 貴弘、大崎 達哉、伊藤 大知、新田見匡、福田 淳二
    • Organizer
      公益社団法人電気化学会第82回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2015-03-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 射出成形技術を模した三次元血管導入骨組織モデルの構築2014

    • Author(s)
      景山達斗、掛川 貴弘、大崎 達哉、伊藤 大知、新田見匡、福田 淳二
    • Organizer
      動物実験代替法学会 第27回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2014-12-07
    • Related Report
      2014 Research-status Report
  • [Presentation] 成形加工プロセスを模倣した骨組織モールディング2014

    • Author(s)
      景山達斗、掛川 貴弘、大崎 達哉、伊藤 大知、新田見匡、福田 淳二
    • Organizer
      日本生物工学会東日本支部 第8回学生発表討論会
    • Place of Presentation
      八王子セミナーハウス
    • Year and Date
      2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] 立体的な肝組織構築の試み2014

    • Author(s)
      福田 淳二、大﨑達哉、武部貴則
    • Organizer
      公益法人化学工学会 第46回秋季大会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 成形加工プロセスを模倣した素早い三次元臓器モールディング2014

    • Author(s)
      景山達斗、掛川 貴弘、大崎 達哉、伊藤 大知、新田見匡、福田 淳二
    • Organizer
      公益法人化学工学会 第46回秋季大会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-09-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 立体組織構築の課題と今後2014

    • Author(s)
      福田淳二
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 電気化学細胞脱離を用いた血管新生の評価用マイクロデバイス2014

    • Author(s)
      篠原礼奈、榎本詢子、新田見匡、福田淳二
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] In situ 架橋ハイドロゲルを用いた三次元臓器モールディング2014

    • Author(s)
      景山達斗、掛川 貴弘、大崎 達哉、伊藤 大知、新田見匡、福田 淳二
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 三次元組織構築のための血管構造モールディング2014

    • Author(s)
      景山達斗、掛川 貴弘、大崎 達哉、伊藤 大知、新田見匡、福田 淳二
    • Organizer
      生物工学会第6回若手研究シンポジウム
    • Place of Presentation
      神戸セミナーハウス
    • Year and Date
      2014-07-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Rapid micromolding of endothelial cell-lined vascular-like structures in insitu crosslinkable hydrogels2014

    • Author(s)
      T. Kageyama, T. Kakegawa, T. Osaki, J. Enomoto,T. Itoand J. Fukuda
    • Organizer
      Termis-EU
    • Place of Presentation
      Genova,&#160;Italy
    • Year and Date
      2014-06-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Electrochemical cell detachment for engineering 3D tissues2014

    • Author(s)
      J. Fukuda
    • Organizer
      JST ERATO International Symposium on 3D Tissue Fabrication
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2014-05-21
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 微細加工を用いた肝細胞スフェロイド培養器2014

    • Author(s)
      福田淳二、穴田貴久、鈴木治
    • Organizer
      第21回HAB研究機構学術年会
    • Place of Presentation
      昭和大学
    • Year and Date
      2014-05-13
    • Related Report
      2014 Research-status Report
  • [Presentation] Electrochemical cell detachment for engineering vascularized tissues2014

    • Author(s)
      J. Fukuda
    • Organizer
      KSBB
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2014-04-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] 酸素透過性三次元細胞培養デバイスの開発2015

    • Author(s)
      穴田貴久、福田淳二、鈴木治
    • Total Pages
      7
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2015 Annual Research Report
  • [Book] 電気化学を用いた細胞操作、三次元ティッシュエンジニアリング2015

    • Author(s)
      大崎達哉、福田淳二
    • Total Pages
      6
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2014 Research-status Report
  • [Book] エヌ・ティー・エス2015

    • Author(s)
      穴田貴久、福田淳二、鈴木治
    • Total Pages
      6
    • Publisher
      酸素透過性三次元細胞培養デバイスの開発、三次元ティッシュエンジニアリング
    • Related Report
      2014 Research-status Report
  • [Book] 血管構造のモールディングによる肝組織形成、三次元ティッシュエンジニアリング2015

    • Author(s)
      大崎達哉、福田淳二
    • Total Pages
      6
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2014 Research-status Report
  • [Book] 成形加工プロセスを模した立体組織作製、マイクロバイオ技術の潮流・実利用・展望2014

    • Author(s)
      景山達斗、掛川貴弘、福田淳二
    • Total Pages
      7
    • Publisher
      生物工学会誌
    • Related Report
      2014 Research-status Report
  • [Book] 細胞培養マイクロデバイス-マイクロスケールでの細胞培養・解析技術2014

    • Author(s)
      福田淳二、掛川貴弘
    • Total Pages
      5
    • Publisher
      化学と生物
    • Related Report
      2014 Research-status Report
  • [Book] 細胞培養マイクロデバイス2014

    • Author(s)
      福田淳二、掛川貴弘
    • Total Pages
      9
    • Publisher
      生物工学会誌
    • Related Report
      2014 Research-status Report
  • [Remarks] 福田研究室

    • URL

      http://www.fukulab.ynu.ac.jp/

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 血管網被包細胞包埋ビーズ及びその製造方法、並びに前記血管網被包細胞包埋ビーズを用いた集積体及びその製造方法2015

    • Inventor(s)
      福田淳二、景山達斗、園山由希江、荒木拓人
    • Industrial Property Rights Holder
      横浜国立大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-10-30
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞包埋ビーズ及びその製造方法2015

    • Inventor(s)
      福田淳二、景山達斗、園山由希江、荒木拓人
    • Industrial Property Rights Holder
      横浜国立大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-09-11
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2022-01-27  

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