Development of DDS system based on the intracellular enviroment responsive aptamers
| Project/Area Number |
26630432
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Kazuhiro 徳島大学, 大学院医歯薬学研究部, 助教 (00644999)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アプタマー / 薬剤放出システム / ヌクレオシド / 細胞環境応答 / 4'-セレノヌクレオチド |
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| Outline of Final Research Achievements |
In this work, we aimed to develop a novel DDS system based on the intracellular enviroment responsive aptamers, called PARCEL（Practical Aptamer Responding to CELlular Environment. In order to complete this project, we prepared 2'-deoxy-4'-selenothymidine 5'-triphosphate (SeTTP). Using the resulting SeTTP, PCR was investigated. As a result, PCR went well when KOD dash DNA polymerase was used in the presence of DMSO. Accordingly, we confirmed the conditions to obtain the desired PARCEL.
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Report
(3 results)
Research Products
(2 results)
