Technical development of single-cell multi-omics

• Project/Area Number: 26640120
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Sasagawa Yohei 国立研究開発法人理化学研究所, 情報基盤センター, 上級センター研究員 (10404344)
• Co-Investigator(Renkei-kenkyūsha): Danno Hiroki 国立研究開発法人理化学研究所, 情報基盤センター, センター研究員 (20610439)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 1細胞RNA-seq / 1細胞エピゲノムシーケンス / single-cell RNA-seq / cell barcoding / 新生RNA / 1細胞 / マルチオミックス / エピゲノム / トランスクリプト / シングルセル

Outline of Final Research Achievements

In this study, we tried to develop technology for simultaneous detection of epigenome and transcriptome from one cell. We separated cytosol and nucleus from a single-cell using pipet tip. We tried to use nucleus for detection of epigenome. Moreover, we succeeded to develop single-cell RNA-seq method, which detect non-poly-A RNA including nascent RNA. Moreover, we tried to develop cell-barcoding method using random primer for high-throughput detection of RNA.

Research Products

• [Journal Article] Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs (2018)
  • Author(s): Tetsutaro Hayashi, Haruka Ozaki, Yohel Sasaqawa, Mana Umeda, Hiroki Danno and ltoshi Nikaido
  • Journal Title: Nature Communications Volume: 9 : 619 Issue: 1 Pages: 1-16
  • DOI: 10.1038/s41467-018-02866-0
  • Peer Reviewed / Open Access
• [Presentation] セルソーターを用いたハイスループット1細胞RNA-seq 法Quartz-Seq2の開発 (2016)
  • Author(s): 笹川 洋平, 團野 宏樹, 海老澤 昌史, 林 哲太郎, 二階堂 愛
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] non-poly-A RNAも検出できる1細胞RNA-seq法の開発と比較 (2015)
  • Author(s): Yohei SASAGAWA, Tetsutaro HAYASHI, Itoshi NIKAIDO
  • Organizer: NGS現場の会 第四回研究会
  • Place of Presentation: つくば国際会議場 (茨城県・つくば市)
  • Year and Date: 2015-07-01