Development of cell culture substratum that restrict morphology of cell aggregate during 3D-morphogenesis

• Project/Area Number: 26650071
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yonemura Shigenobu 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, チームリーダー (60192811)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 上皮細胞 / 細胞接着 / 形態形成 / 微細加工 / 三次元培養 / PDMS / 細胞集合 / マイクロファブリケーション

Outline of Final Research Achievements

To restrict the initial morphology of epithelial cell aggregates is the purpose of this study. I designed a metal mold to make PDMS which has V-shaped grooves. The initial morphology of epithelial cell aggregates was successfully restricted by seeding cell onto this PDMS. By tuning the depth or width of V-shape grooves, observation of several number of V-shaped grooves was possible. As a result, during cell adhesion, the aspect ratios of the cell aggregates became low quickly in a special time window, indicating that epithelial cells have a mechanism to correct their aggregate shape to ball during their adhesion.

Research Products

• [Journal Article] Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. (2016)
  • Author(s): Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M.
  • Journal Title: J Cell Biol. Volume: 212 Issue: 4 Pages: 409-423
  • DOI: 10.1083/jcb.201507046
  • Peer Reviewed / Open Access
• [Journal Article] Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells. (2016)
  • Author(s): Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.
  • Journal Title: Nat Commun. Volume: 7 Issue: 1 Pages: 10351-10351
  • DOI: 10.1038/ncomms10351
  • NAID: 120006535047
  • Peer Reviewed / Open Access
• [Journal Article] Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration. (2016)
  • Author(s): Shirai H, Mandai M, Matsushita K, Kuwahara A, Yonemura S, Nakano T, Assawachananont J, Kimura T, Saito K, Terasaki H, Eiraku M, Sasai Y, Takahashi M.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 113 Issue: 1
  • DOI: 10.1073/pnas.1512590113
  • Peer Reviewed / Open Access
• [Journal Article] 細胞間メカノトランスダクション (2016)
  • Author(s): 鳥澤久美子, 米村重信
  • Journal Title: 生体の科学 Volume: 67 Pages: 137-141
• [Journal Article] Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off. (2015)
  • Author(s): Shinohara K, Chen D, Nishida T, Misaki K, Yonemura S, Hamada H.
  • Journal Title: Dev Cell. Volume: 35 Pages: 236-246
• [Presentation] お互い接着しない上皮細胞を使って極性形成機構を探求する (2016)
  • Author(s): 米村重信
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 2016-06-17
  • Invited