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Exploration of unknown genetic function in subseafloor biosphere by Substrate-Induced Gene Expression approach

Research Project

Project/Area Number 26650169
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

MORONO Yuki  国立研究開発法人海洋研究開発機構, 高知コア研究所, グループリーダー代理 (30421845)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsSubseafloor biosphere / Gene function / 海底下生命圏 / 未知機能遺伝子 / 基質誘導 / 遺伝子発現 / 遺伝子機能 / 海底下 / 未知微生物
Outline of Final Research Achievements

In this study, we aimed to explore genetic function of microbial life inhabited in subseafloor biosphere. For searching unknown microbial genetic function, we employed substrate-induced gene expression (SIGEX) approach that relies gene expression induced by exposure of substrate.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 6 results) Book (2 results)

  • [Journal Article] A modified SDS-based DNA extraction method for high quality environmental DNA from2016

    • Author(s)
      Vengadesh Perumal Natarajan, Xinxu Zhang, Yuki Morono, Fumio Inagaki and Fengping Wang
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 986-986

    • DOI

      10.3389/fmicb.2016.00986

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Ecophysiology of Zetaproteobacteria Associated with Shallow Hydrothermal Iron-Oxyhydroxide Deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan2015

    • Author(s)
      HOSHINO TATSUHIKO, Kuratomi Takashi,MORONO YUKI,Hori Tomoyuki,Oiwane Hisashi,Kiyokawa Shoichi,INAGAKI FUMIO
    • Journal Title

      Frontiers in Microbiology

      Volume: 6 Pages: 1554-1554

    • DOI

      10.3389/fmicb.2015.01554

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring deep microbial life in coal-bearing sediments down to ~2.5km below the seafloor2015

    • Author(s)
      INAGAKI FUMIO, Kai-Uwe Hinrichs,KUBO YUSUKE,Marshall W. Bowles,Verena B. Heuer,Wei-Li Hong,HOSHINO TATSUHIKO,IJIRI AKIRA,IMACHI HIROYUKI,ITO MOTOO,KANEKO MASANORI,Mark A. Lever,Lin Yu-Shin,Barbara A. Methe,Morita Sumito,MORONO YUKI,et al.
    • Journal Title

      SCIENCE/AAAS/

      Volume: 349 Issue: 6246 Pages: 420-424

    • DOI

      10.1126/science.aaa6882

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Intact preservation of environmental samples by freezing under an alternating magnetic field2015

    • Author(s)
      Morono, Y., Terada, T., Yamamoto, Y., Xiao, N., Hirose, T., Sugeno, M., Ohwada, N., Inagaki F
    • Journal Title

      Environ. Microbiol. Rep.

      Volume: 7 Issue: 2 Pages: 243-251

    • DOI

      10.1111/1758-2229.12238

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Origins of lithium in submarine mud volcano fluid in the Nankai accretionary wedge2015

    • Author(s)
      Nishio, Y., Ijiri, A., Toki, T., Morono, Y., Tanimizu, M., Nagaishi, K., and Inagaki, F.
    • Journal Title

      Earth Plant. Sci. Lett.

      Volume: 414 Pages: 144-155

    • DOI

      10.1016/j.epsl.2015.01.018

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 海底下環境における微生物生命圏とその生態2014

    • Author(s)
      諸野祐樹
    • Journal Title

      Journal of Japanese Society for Extremophiles

      Volume: 13 Pages: 4-10

    • Related Report
      2014 Research-status Report
  • [Presentation] Technological breakthroughs in search of the deep subseafloor biosphere2016

    • Author(s)
      諸野 祐樹、寺田 武志、伊藤 元雄、稲垣 史生
    • Organizer
      Japan Geoscience Union Meeting 2016
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-05-22
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 海底下に広がる巨大生命圏とその探索ー最先端技術で生命の実態にせまるー2016

    • Author(s)
      諸野祐樹
    • Organizer
      日本農芸化学会中四国支部創立15周年記念第22回若手研究者シンポジウム
    • Place of Presentation
      高知大学農学部(高知県南国市)
    • Year and Date
      2016-04-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 深海底コア試料を用いたSIGEX法によるD-アミノ酸/希少糖代謝酵素遺伝子の 網羅的探索2015

    • Author(s)
      添田 愛理沙, 諸野 祐樹,寺田 武志,稲垣 史生,芦内 誠,若松 泰介
    • Organizer
      日本農芸化学会2015年度中四国・西日本支部合同大会
    • Place of Presentation
      愛媛大学 (愛媛県松山市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 深海底コア試料を用いたSIGEX法によるレアメタル応答遺伝子の網羅的探索と 解析2015

    • Author(s)
      尾崎 和弘, 諸野 祐樹,寺田 武志,西川 聡美,北村 萌,稲垣 史生,芦内 誠,若松 泰介
    • Organizer
      日本農芸化学会2015年度中四国・西日本支部合同大会
    • Place of Presentation
      愛媛大学 (愛媛県松山市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Technical developments to explore sedimentary biosphere: current status, limitation, and future perspectives2015

    • Author(s)
      MORONO YUKI,
    • Organizer
      CDEBI Sediment Workshop
    • Place of Presentation
      University of Southern California (USA)
    • Year and Date
      2015-06-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 海底下で太古の生命を探せー最先端技術で迫る海底下深部生命とその姿,2014

    • Author(s)
      諸野祐樹
    • Organizer
      高知大学自主セミナー
    • Place of Presentation
      高知大学農学部、高知県南国市
    • Year and Date
      2014-11-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] エアロゾルDNAフリー環境の構築とその評価2014

    • Author(s)
      諸野祐樹、星野辰彦、鈴木剛人、佐藤卓広、湯浅久史、久保田裕仁、寺田武志、稲垣史生
    • Organizer
      極限環境生物学会年会
    • Place of Presentation
      今帰仁村コミュニティセンター、沖縄県国頭郡
    • Year and Date
      2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 基質誘導型遺伝子発現解析による海底下微生物の機能遺伝子探索2014

    • Author(s)
      諸野祐樹、寺田武志、星野辰彦、二神泰基、稲垣史生
    • Organizer
      環境微生物系学会合同大会
    • Place of Presentation
      浜松アクトシティ、静岡県浜松市
    • Year and Date
      2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] Intact preservation of environmental samples by freezing under an alternating magnetic field2014

    • Author(s)
      Morono Y.
    • Organizer
      Fourth C-DEBI Annual Meeting
    • Place of Presentation
      Marina, California, USA
    • Year and Date
      2014-10-03
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 熱アルカリ細胞溶菌法による海底下微生物群集構造解析2014

    • Author(s)
      諸野祐樹、寺田武志、星野辰彦、稲垣史生
    • Organizer
      日本地球惑星科学連合2014年大会
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2014-04-30
    • Related Report
      2014 Research-status Report
  • [Presentation] 海底下に広がる生命圏,2014

    • Author(s)
      諸野祐樹
    • Organizer
      統合国際深海掘削計画10年の成果一般公開シンポジウム、深海を掘る
    • Place of Presentation
      国立科学博物館、東京都台東区
    • Year and Date
      2014-04-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] Earth and Life Processes Discovered from Subseafloor Environments: A Decade of Science Achieved by the Integrated Ocean Drilling Program (IODP)2014

    • Author(s)
      Morono, Y., Kallmeyer, J.
    • Total Pages
      325
    • Publisher
      Elsevier
    • Related Report
      2014 Research-status Report
  • [Book] “Life in Extreme Environments: Microbial Life in the Deep Biosphere”, Chapter 52014

    • Author(s)
      Morono, Y., Ito, M., and Inagaki, F.
    • Total Pages
      822
    • Publisher
      DeGruyter
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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