| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In order to control aquatic pests, a system for preparing an insoluble protein toxin was constructed by fusing a cell recognition site, a cytotoxic site and a protein crystallization tag. Insolubilizing increases the concentration of toxin, it is possible to cause a target organism to take a lethal dose. insolubilizing also suppress dilution and loss of toxin in water, and unnecessary environmental contamination. Cry46Ab derived from soil bacteria showed lethal activities against mosquito insters and the apple snail, but not against medaka. Therefore, insoluble toxin granules of Cry46Ab with a crystallization tag was prepared, and it was confirmed that this fusion protein retained lethal activities.
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