Development of an aquatic pest control with crystalline toxin modified cell recognition sites
| Project/Area Number |
26660044
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Kindai University |
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Principal Investigator |
TAKEBE So 近畿大学, 生物理工学部, 教授 (20227052)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 微生物農薬 / 水生有害生物 / 選択毒性 / タンパク質不溶化 / Bacillus thuringiensis / 生物農薬 / タンパク質の不溶化 |
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| Outline of Final Research Achievements |
In order to control aquatic pests, a system for preparing an insoluble protein toxin was constructed by fusing a cell recognition site, a cytotoxic site and a protein crystallization tag. Insolubilizing increases the concentration of toxin, it is possible to cause a target organism to take a lethal dose. insolubilizing also suppress dilution and loss of toxin in water, and unnecessary environmental contamination. Cry46Ab derived from soil bacteria showed lethal activities against mosquito insters and the apple snail, but not against medaka. Therefore, insoluble toxin granules of Cry46Ab with a crystallization tag was prepared, and it was confirmed that this fusion protein retained lethal activities.
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Report
(4 results)
Research Products
(8 results)
