Combination Effect of elevated O3 and CO2 on growth of larch species and species diversity of ectomycorrhizae

• Project/Area Number: 26660119
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Hokkaido University
• Principal Investigator: KOIKE Takayoshi 北海道大学, (連合)農学研究科(研究院), 教授 (10270919)
• Co-Investigator(Kenkyū-buntansha): TAMAI Yutaka 北海道大学, 大学院農学研究院, 准教授 (50281796) ; WATANABE Makoto 東京農工大学, 大学院農学研究院, 准教授 (50612256) ; 佐藤 冬樹 北海道大学, 北方生物圏フィールド科学センター, 教授 (20187230)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: カラマツ属 / 無機栄養 / 外生菌根菌 / 光合成 / 種多様性 / 感染率 / 高CO２ / 高オゾン / 対流圏オゾン / 高CO2環境 / グイマツ雑種F1 / リン鉱石枯渇 / 共生関係 / OTC / 高CO2 / 地表付近オゾン / 共生菌類

Outline of Final Research Achievements

We studied colonization of ectomycorrhiza (ECM) fungi and the species abundance of a hybrid larch F1 (Larix gmelinii var. japonica × L. kaempferi) with elevated CO2 and ozone (O3). Two-year-old seedlings were planted in an Open-Top-Chamber (OTC) system with 4 gas fumigations: Control (< 6 nmol/mol), O3 (60 nmol/mol), CO2 (600 μmol/mol), and CO2+O3. After 2 growing seasons, ECM colonization and root biomass were positively affected by elevated CO2. Ozone impaired ECM colonization rate and species richness, and reduced stem biomass. However there was no clear inhibition on photosynthetic capacity by O3. Concentration of most elements (Al, Fe, Mo, P) in needles was reduced by O3 but some increased in roots (K, Mg), which may lead to distinct alteration of ECM abundance. We found no effects of combined fumigation on all parameters except P concentration in needles. Tolerance to O3 shown in hybrid larch F1 might be potentially related to ECM symbiosis changes.

Research Products

• [Int'l Joint Research] 河北農業大学(中国)
• [Int'l Joint Research] CAS 生態・環境研究センター(中国)
• [Journal Article] Fine root turnover of Japanese white birch (Betula platyphylla var. japonica) grown under elevated CO2 in northern Japan (2016)
  • Author(s): Wang, XN., S. Fujita, T. Nakaji, M. Watanabe, F Satoh and T. Koike
  • Journal Title: Trees Volume: 30 Pages: 363-374
  • NAID: 120005981523
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Impact of elevated CO2 on root traits of a sapling community of three birches and an oak: A free-air-CO2 enrichment (FACE) in northern Japan. (2016)
  • Author(s): Agathokleous E, Watanabe M, Nakaji T, Wang XN, Satoh F, and Koike T.
  • Journal Title: Trees Volume: 30 Pages: 353-362
  • NAID: 120005981521
  • Peer Reviewed
• [Journal Article] Effects of CO2 and/or O3 on the interaction between root of woody plants and ectomycorrhizae. (2016)
  • Author(s): Wang, XN, Agathokleous, E., Qu, L.Y., Watanabe, M., and Koike, T.
  • Journal Title: Effects of CO2 and/or O3 on the interaction between root of woody plants and ectomycorrhizae. Volume: 73
  • Peer Reviewed / Open Access
• [Journal Article] Ectomycorrhizal colonization and growth of the hybrid larch F1 under elevated CO2 and O3. (2015)
  • Author(s): Wang, XN, L Qu, Q Mao, M Watanabe, Y Hoshika, A Koyama, K Kawaguchi, Y Tamai and T Koike
  • Journal Title: Environmental Pollution Volume: 197 Pages: 116-126
  • NAID: 120005602512
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ecophysiology of deciduous trees native to Northeast Asia grown under FACE (Free Air CO2 Enrichment) (2015)
  • Author(s): Koike, T., Watanabe, M., Watanabe, Y., Agathokleous, E., Mao, QZ., Eguchi, N., Takagi, K., Satoh, F., Kitaoka, S., and Funada, R.
  • Journal Title: Journal of Agricultural Meteorology Volume: 71 Pages: 174-184
  • NAID: 130005098166
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Wild plant species as subjects in O3 research. (2015)
  • Author(s): Agathokleous, E., Saitanis, C.J., Satoh, F. and Koike, T.
  • Journal Title: Eurasian J. Forest Research Volume: 18 Pages: 1-36
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ectomycorrhizal colonization and growth of the hybrid larch F1 under elevated O3 and CO2. (2015)
  • Author(s): Wang, X., Qu, L., Mao, Q., Watanabe, M., Hoshika, Y., Koyama, A., Kawaguchi, K., Tamai, Y. and Koike, T.
  • Journal Title: Environmental Pollution Volume: 197 Pages: 116-126
  • DOI: 10.1016/j.envpol.2014.11.031
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] カラマツのパートナー (2015)
  • Author(s): 小池孝良
  • Journal Title: 樹木医学 Volume: 19(1) Pages: 60-61
  • NAID: 130007481006
  • Peer Reviewed
• [Presentation] Sustainable forest production with hybrid larch F1 under changing environment: Prediction with a free-air system and OTC (2016)
  • Author(s): Koike, T, Wang XN, Qu LY, Fujita S, Sugai T, Shi C and Watanabe T
  • Organizer: Nankai University-HokkaidoUniversity conference
  • Place of Presentation: 天津、中国、南開大学
  • Year and Date: 2016-03-28
• [Presentation] Sustainable forestry production under changing environment (2015)
  • Author(s): Takayoshi Koike, Evgenios Agathokleous, Cong Shi, Xiaona Wang & Toshihiro Watanabe
  • Organizer: The 2nd Mynamer-Japan symposium
  • Place of Presentation: Pathein,Myanmer, Pathein University
  • Year and Date: 2015-12-05
• [Remarks] 発起道大学造林学・小池孝良
  • URL: http://www.agr.hokudai.ac.jp/fres/silv/index.php?%BE%AE%C3%D3%B9%A7%CE%C9